الفهرس 
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Abstract
The present study was designed to throw spot light upon the bacteriological status of RTE organ meats in Sohag city. A total of 120 random samples of RTE organ meats (cooked liver sandwiches, cooked spleen dishes, cooked lung dishes and cooked tripe dishes) collected from different restaurants in Sohag city represented as 30 samples each.
The results of APC revealed that the mean values in the examined samples of cooked liver, spleen, lung and tripe were 3.3, 3.04, 3.8 and 3.78 log.CFU/g, respectively.
The results of this study showed that the prevalence of Staph. aureus was 15.8% (19/120). Concerning staph. aureus count, data revealed the mean values were 1.1, .5, 1.6, 1.7 log. CFU/g of liver, spleen, lung and tripe, respectively. Enterotoxigenic Staph. aureus was detected in 4 samples (21.05%) of the coagulase positive Staph. aureus strains. The enterotoxins produced were identified as 2 strains of SE A in liver sandwiches, one SE C (20%) of lung samples and one SE A & SE D (33.3%) of spleen samples. Virulence genes of Staph. aureus which indicated by mPCR were 19/120 (15.8%) positive Staph. aureus strains for nuc gene while 3/120 (2.5%) positive for mecA gene.
On the other hand, the bacteriological examination of the examined samples revealed that 2 samples of cooked liver were positive for Salmonella spp. with a percentage of 6.6 % which were identified as 1 (3.3%) S. Enteritidis and 1 (3.3%) and S. Molade (3.3%).
Multiplex PCR was used to detect the simultaneous presence of invA, stn and hilA in salmonella strains that were isolated in this study as virulence genes, S. Enteritidis strains which were isolated from the examined cooked liver sandwitches were positive invA, stn and hilA, while, S. Molade were positive for invA and hilA.
Public health importance of the isolated microorganisms, Staph. aureus and Salmonella Enteritidis. as well as methods for protecting consumer were discussed.