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Abstract Summary Based on the information presented in this thesis ,we make the following summary: P.aeruginosa is an ubiquitous organism which has emerged as a major threat in hospital environment. P. aeruginosa is the most commonly isolated bacterium colonizing severe burns and wound infections . The pathogenesis of P. aeruginosa in burn has been extensively studied and proven to be a multifactorial process mediated by virulence factors including exotoxin A gene . The aim of this study was to detect P.aeruginosa from burn infection . MDR Pseudomonas isolates were taken and PCR was done on them to detect the distribution of EXO-A gene among them. The present study was carried out in The Medical Microbiology and Immunology Department, Faculty of Medicine, Tanta University and Central Research Laboratory of Tanta University, on 200 patients admitted to burn units of surgery department at Tanta university hospitals during the period of research from February 2019 to November 2020. All patients were subjected to thorough history taking and full clinical examination. 200 burn swabs were collected under complete aseptic condition. All samples were cultured on ordinary media (Nutrient agar) and differential |