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Abstract In this study, 100 diarrheic chicken birds (5 samples per bird; liver, gall bladder, spleen, cloacal swab and intestinal content) and 100 random rectal swabs taken from diarrheic calves were obtained randomly from different farms in Kafrelsheikh governorate. Collected samples were examined for the presence of S. enterica and E. coli O157:H7. Biochemical and serological identification were applied. Biochemical and serological examination revealed that the prevalence of S. enterica was 5%(10 isolates) from all samples and E. coli O157:H7 was 1%(1 isolate)from diarrheic calves samples. Serlogically, the 10 isolates serologically typed as 3 isolates belonged to S. Kentucky (2 isolates from chickens and 1 from calves), 3 isolates belonged to S. Typhimurium (3 isolates from chickens), 4 isolates belonged to S. Enteritidis (3 isolates from chickens and 1 isolate from calves).In vitro sensitivity of S. enterica isolates to various antimicrobial agents revealed that the prevalence of resistant to β-lactamase penicillins Amoxicillin (9/10)(90%), Ampicillin ( 10/ 10)(100%), cephalosporines Cefotaxime ( 1/10)(10%), ceftriaxone (0/10)(0%), Quinolones antibiotic ,Ciprofloxacin (1/10)(10%), nalidixic acid (6/10)(60%), Enrofloxacin (2/10)(20%),.Then molecular characterization of β-lactamase-encoding genes in Salmonella isolates, The blaTEM was identified by PCR in 4 of 10 isolates (40 %); S. Enteritidis (2 isolates), S. Kentucky (2 isolates) while the blaIMP, blaVIM, and blaSHV could not be identified in any of the 4 isolates. also, PCR-screening results detected Amplified products of Variable integrons and int1 amplicon (491 bp) 100% (4/4). The virulence genes in isolated E. coli O157: H7 By using PCR screening was positive for stx1 and stx2 but negative for hlyA also amplified products of Variable integrons detected. |