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العنوان
Studies on Antibiotic Resistant Food Poisoning Microorganisms in Some Dairy Products./
المؤلف
Fathalla, Eman kamal Mohamed
هيئة الاعداد
باحث / Eman kamal Mohamed Fathalla
مشرف / Abdel-Rahman Mahmoud El-Bagory
مشرف / Heba Hussein Shelaby
مشرف / Seham Nazeh Homoda
الموضوع
dairy microbiology. food microbiology.
تاريخ النشر
2019
عدد الصفحات
180p. ;
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
28/9/2019
مكان الإجازة
جامعة مدينة السادات - المكتبة المركزية بالسادات - (Department of Food Hygiene & Control)
الفهرس
Only 14 pages are availabe for public view

from 153

from 153

Abstract

Two hundred samples, 50 each of Kareish cheese, Ras cheese, Ice cream and Milk powder were collected from supermarkets, dairy shops and street vendors in El- Gharbia governorate for microbiological analysis.
Microbiological examination of the examined samples revealed that E. coli could be detected in 32, 24, 18 and 10% of examined Kareish cheese, ras cheese, ice cream and milk powder samples, respectively.
S.aureus could be detected in 30, 22, 16 and 8% of examined Kareish cheese, ras cheese, ice cream and milk powder samples, respectively.
B.cereus could be detected in 8, 14, 10 and 20% of examined Kareish cheese, ras cheese, ice cream and milk powder samples, respectively.
Enterobacter sakazakii could not be detected in all the examined samples.
Concerning the antibiotic sensitivity test all the isolated isolates were tested against Gentamicin (120μg), Erythromycin (15μg), E-moxclav (30), Ampicillin (10μg), Ciprofloxacin (5μg), Doxycyclin (30), Cefazone (75), Sulphamethaoxazole-trimethoprim (25) and Vancomycin (30).
E.coli isolates were highly resistant to Vancomycin (30μg) and Sulphamethaoxazole-trimethoprim (25μg), while low resistant were to Ciprofloxacin (10μg) and Doxycyclin (30μg). S.aureus isolates recorded high resistant against Cefazone (75μg), and low resistant against Ciprofloxacin (10μg). B.cereus isolates were highly resistant to Cefazone (75μg), while the low resistant was to Gentamicin (120μg).
Pcr for some antibiotic resistant isolates revealed that that in E.coli presence of blaTEM (ampicillin resistant strains), sul1 (sulphamethoxazole resistant) and dfrA (trimethoprim resistant) genes in all the strains examined by PCR. B.cereus revealed that TetA(tetracyclin reistant) and bla (beta lactamase resistant) resistant genes were found in all the examined samples while ermA (erythromycin resistant) gene were not isolated. S.aureus showed that mecA (methicillin resistant) gene and vanA (vancomycin resistant) gene were found in only one strain, while blaZ (beta lactamase resistant)gene was found in all the examined samples.
Investigation the effect of Pomegranate Fruit peels and Moringa oleifera leaves ethanolic extracts at concentration (1& 2% from each extract) on sensory evaluation and their inhibitory effect on EHEC (O111: H2) in yoghurt during storage, proved to all treatments and control yoghurt were acceptable and the count of E. coli was significally reduced during the periods of incubation.