الفهرس 
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Abstract
This study was done at Menoufia province during 2015; to study the seroprevalence of brucellosis among cattle population in different centers of the province using RBPT as screening test. The effect of some risk factors on the prevalence of brucellosios such as locality, breed and age of cattle. As well as evaluation of the specificity and sensitivity of RBPT, IC assay and cELISA against CFT test were determined. Molecular detection of brucella using PCR and bacteriological isolation of brucella microorganism from tissue samples were done followed by phenotypic and molecular typing of the isolated bacteria.
The overall seroprevalence of bovine brucellosis in Menoufia province among cattle population during 2015 using RBPT as screening test was 0.717% (91 positive out of 12681), while the apparent prevalence was 1.5%.
The seroprevalence of bovine brucellosis was recorded in 9 centers of Menoufia province. The highest percentage of apparent and true prevalence of bovine brucellosis were reported in Menouf (3.5%) , (3%) and Qusena (3%), (2.6%) respectively, while the lowest percentage of apparent and true prevalence of bovine brucellosis was Shebin Elkom (0.9%) and (0%). In the same side Tala was (1.3%), (0.4), Brekit Elsabaa (1.2%), (0.3%), Ashmoun (1.2%), (0.3%), Elshohada (1%), (0%) and Elbagour (1%), (0%) lies in the middle. With no seropositives reactors recorded in Sadat center.
It was found that Cows in Quesna and Menouf were found to be of highest significant risk of getting brucellosis other than other districts. While cows in Elshohada were at lower risk of getting Brucella infection more than other districts.
The seroprevalence of brucellosis in cattle under 1 year of age was 0.354%, in animal from 1 to 3 years of age was 1.226% and in animals over 3 years of age was 2.777%. Logistic regression for the measuring the association between the age and seropositivity found that old animals are at higher risk of getting brucellosis infection.
The seroprevalence of brucellosis among local breed of cattle was 1.28% and among Holstein Friesian cattle was 1.57%. Logistic regression for the measuring the association between the breed and seropositivity found that Frisian breed was the highest prevalence of bovine brucellosis while the native breed cattle was the lowest for getting brucella infection. The odd ration among Frisian animal was 2 times more than native breed cattle.
A total of 100 serum samples collected from suspected cases were examined by the 4 different serological tests RBPT, cELISA, IC Assay and CFT; with 91%, 60%, 91% and 88% positive results respectively. The sensitivity of RBPT, cELISA and IC Assay in comparison with CFT was estimated at 100%, 1000% and 68.2%, respectively. On the other hand, the specificity for the 3 serological tests was estimated at 75%, 75% and 100%, respectively.
Bacteriological isolation of brucella species from the tissue specimens (uterus and/or lymph nodes) had been done successfully from 6 tissue specimens out of 20 examine samples. Phenotypic and bruce ladder typing of the single brucella isolates indicated that the isolate was Brucella melitensis biovar 3.
Molecular detection of brucella species directly from the tissue samples (uterus and/or lymph nodes) using PCR targeting (Immunodominant antigen, gene bp26) had been on 20 tissue samples. The PCR generated product of 450 bp from 16 tissues specimens.