الفهرس 
Steroid bioconversion reactionsOnly 14 pages are availabe for public view
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Abstract
This study was carried out on microbial bioconversion of phytosterols to some important steroidal hormones. A total thirty water samples were collected these samples include ten samples from agriculture wastewater in El-Qalyubyia Governorate, and twenty samples from sewage water in El-Beheira Governorate, Egypt. Twelve bacterial isolates were purified on nutrient agar slants and incubated for 24 h at 35 c then maintained on slants and stored at 4 c with monthly subculture for maintenance. Isolates were screened for the active bioconversion of β, sitosterol results showed that four isolates (2, 4, 8, 10) produced 4-androstene-3, 17-dione AD) and 1,4 androsta-diene-3,17-dione (ADD) therefore it was selected for the quantitative determination of the products. Four bacterial isolates (2, 4, 8, 10) were identified using Gram staining and some biochemical tests (catalase, oxidase, coagulase, indole production, urease then genetic identification made by 16S rRNA gene sequence for two bacterial isolates (2 and 4). Bacterial isolates 2 and 4 were identified as Ochrobactrum anthropi strain ATCC 49188 and Kocuria atrinae strain P30T.