الفهرس | Only 14 pages are availabe for public view |
Abstract This In-Vitro study was conducted to evaluate the penetration ability of two different types of sealers; a calcium silicate based root canal sealer (Endosequence BC Sealer) and an epoxy based resin sealer (AH Plus sealer). Furthermore; to assess the antimicrobial ability of these two sealers over a thirty day period in eradicating the bacterial contamination inside the dentinal tubules of the root canal, using an in vitro model contaminated with Enterococcus faecalis. Seventy five freshly extracted single rooted teeth were selected for this study. Standardized root segments of a length of seven mm were prepared. 56 specimens underwent antimicrobial testing while 18 specimens underwent penetration ability testing. For antimicrobial testing, following smear layer removal and sterilization the 56 specimens were infected with Enterococcus faecalis (ATCC 29212) suspension using centrifugation and incubation for three weeks. The specimens were then randomly allocated into three groups with 18 specimens in each group according to the sealer to be tested. The specimens were obturated using cold lateral obturation and embedded one mm into a preprepared Brain Heart infusion agar plate. Each group contained three subgroups with six specimens in each subgroup according to the testing period performed (one day, seven days and thirty days). After each testing period, dentin powder was collected from within the canal lumen using sequential removal via the use of peeso reamers sizes three, four, five and six. Proportions of remaining bacteria inside the dentinal tubules at each ISO Size after exposure to the root canal sealers were assessed by Colony forming units (CFU) count on blood agar plates. 135 For penetration ability testing, following smear layer removal the specimens were randomly divided into three main groups with 6 specimens per group according to the sealer to be tested. The specimens were obturated using cold lateral obturation and incubated for two weeks at 37˚C in 100% relative humidity. Following the incubation period the specimens were then transversely sectioned and prepared to be observed under the scanning electron microscope. Minimum and maximum sealer penetration depths were measured and tabulated. Results were tabulated and statistically analyzed using suitable statistical tests. |