الفهرس 
Review of LiteratureOnly 14 pages are availabe for public view
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Abstract
The fast growing of microorganisms resistant to multi antimicrobial drugs have vast impact on mortality and human healthcare. The antimicrobial peptides are highly active against most microbes such as Gram-positive and Gram-negative bacteria, fungi, viruses by disruption of cell membrane. The plants have been used for medicinal purpose as old as the history of mankind. Thus extraction and characterization of several active phytocompounds from the medicinal plants is the basis for the formation of some high activity profile drugs. Further, the phytochemical contents and pharmacological actions. Moringa plants having medicinal potential remain UN assessed by rigorous scientific research to define efficacy and safety. The leaves, fruits, flowers and immature pods of this tree are used as a highly nutritive vegetable in many countries. M. oleifera leaves has been reported to be a rich source of -carotene, protein, vitamin C, calcium and potassium and act as a good source of natural antioxidants.
1) one hundred isolates collected from different patients suffered from different diseases from ZUH, and from these isolates only 32 isolates showed blood hemolysis, these 32 isolates experimented against buffalo whey protein and moringa leaves and seeds extracts and the most sensitive isolates identified by biochemical testes and molecular identification by 16SrRNA which identified as Staph. pasteuri MN368257.
2) In this study buffalo whey protein was hydrolyzed with papain, trypsin and pepsin enzymes to liberate the antibacterial peptides. The extent of protein degradation by trypsin was estimated by assessing the degree of hydrolysis (DH) and SDS-PAGE analysis. The hydrolysate obtained after 180 min. degradation had the highest DH (23%) in comparison with those obtained after 60 and 120 min (9% and 18%, respectively).
3) Molecular mass of BWP as obtained by SDS-PAGE as affected by trypsin at different times 0,1,2 and 3hr. showed two major peaks Size exclusion chromatography fraction (SEC-F1, SEC-F2 and SEC-F3) at 2hr., peaks were collected, lyophilized and assessed for their antibacterial activity.
4) Molecular mass of three fraction of BWP as obtained by UREA-PAGE, the faster migration of SEC-F2 on urea-page into cathode direction than hydrolyzed protein, SEC-F1 and SEC-F3 which may refer to bigger positive charge.
5) The FT-IR spectrum of SEC-F2 fraction of BWP shows the presence of characteristic functional groups of alcohol, hydroxyl, alkane, aldehydes and alkenes groups, nitro, aromatic amines, aliphatic amines and alkyl halides compounds.
6) Buffalo whey protein tested for antibacterial activity against Staph. pasteuri, SEC-F2 of BWP gave the maximum bacterial inhibition than SEC-F1 and SEC-F3.
7) The minimum inhibitory concentration of SEC-F2 was found to be 200 µg/ml against Staph. pasteuri.
8) Evaluation of antibacterial activities of mixture of SEC-F2 with vancomycin, tetracyclin and amoxicillin has less effect on Staph. pasteuri than SEC-F2 without antibiotics.
9) TEM images of SEC-F2 treated bacteria show various signs of cellular deformation, indicating on direct disruptive action of SEC-F2 on the cell wall and cell membrane.
10) In the present study leaves and seeds of M. oleifera were collected from Zagazig, identified and with successive extraction method with hexane, ethanol and water extracts were screened for their antibacterial. Ethanolic extract of leaves and seeds of M. oleifera were screened against the tested identified bacteria.
11) Antibacterial activity of these extracts against Staph. pasteuri showed that ethanolic extract of moringa leaves and seeds has the most effective extract.
12) The minimum inhibitory concentration of moringa leaves ethanolic extract was found to be 50 µg/ml and seeds ethanolic extract was found to be 400 µg/ml against Staph. pasteuri.
13) Quantitative inhibition of Staph. pasteuri at O.D.600by ethanolic extract of moringa leaves and seeds showed inhibition in bacterial growth compared to control.
14) In this study moringa leaves and seeds inhibit Staph. pasteuri at lowest concentration 50g/ml for leaves and 400 g/ml for seeds. A mixture of aqueous ethanolic moringa leaves and seeds extract with vancomycin, tetracycline and amoxicillin has less effect on Staph. pasteuri than leaves and seeds without antibiotics.
15) Detection and purification of antibacterial compounds by paper chromatography to separate active substances from moringa leaves and seeds, these substances detected by FT-IR, UV and GC-Mass. FT-IR for leaves and seeds showed that leaves and seeds contains functional groups of alcohol, hydroxyl, alkane, aldehydes and alkenes groups, aromatic amines, aliphatic amines and alkyl halides compounds, and UV for leaves and seeds showed a peak centered near to 300nm.and GC-Mass for leaves showed seven principle peaks that correspond to 19 active compounds and 11 active compounds for seeds. This showed that the most active compounds has antimicrobial activity are alcoholic, olefene, ketonic derivatives, esters and fatty esters compounds.
16) TEM images of moringa seeds and leaves against Staph. pasteuri show various signs of cellular deformation, indicating on direct disruptive action of moringa seeds and leaves on the cell wall and cell membrane.
17) Estimation of total phenolic, flavenoids and antioxidants compounds of moringa leaves and seeds ethanolic extract showed the highest level content of phenolic, flavenoids and antioxidants compared to control.
18) AgNPs derived from moringa leaves ethanolic extract and SEC- of BWP has less activity against Staph. pasteuri compared to moringa and SEC-F2 as raw material without nanoparticles.
The fast growing of microorganisms resistant to multi antimicrobial drugs have vast impact on mortality and human healthcare. The antimicrobial peptides are highly active against most microbes such as Gram-positive and Gram-negative bacteria, fungi, viruses by disruption of cell membrane. The plants have been used for medicinal purpose as old as the history of mankind. Thus extraction and characterization of several active phytocompounds from the medicinal plants is the basis for the formation of some high activity profile drugs. Further, the phytochemical contents and pharmacological actions. Moringa plants having medicinal potential remain UN assessed by rigorous scientific research to define efficacy and safety. The leaves, fruits, flowers and immature pods of this tree are used as a highly nutritive vegetable in many countries. M. oleifera leaves has been reported to be a rich source of -carotene, protein, vitamin C, calcium and potassium and act as a good source of natural antioxidants.
1) one hundred isolates collected from different patients suffered from different diseases from ZUH, and from these isolates only 32 isolates showed blood hemolysis, these 32 isolates experimented against buffalo whey protein and moringa leaves and seeds extracts and the most sensitive isolates identified by biochemical testes and molecular identification by 16SrRNA which identified as Staph. pasteuri MN368257.
2) In this study buffalo whey protein was hydrolyzed with papain, trypsin and pepsin enzymes to liberate the antibacterial peptides. The extent of protein degradation by trypsin was estimated by assessing the degree of hydrolysis (DH) and SDS-PAGE analysis. The hydrolysate obtained after 180 min. degradation had the highest DH (23%) in comparison with those obtained after 60 and 120 min (9% and 18%, respectively).
3) Molecular mass of BWP as obtained by SDS-PAGE as affected by trypsin at different times 0,1,2 and 3hr. showed two major peaks Size exclusion chromatography fraction (SEC-F1, SEC-F2 and SEC-F3) at 2hr., peaks were collected, lyophilized and assessed for their antibacterial activity.
4) Molecular mass of three fraction of BWP as obtained by UREA-PAGE, the faster migration of SEC-F2 on urea-page into cathode direction than hydrolyzed protein, SEC-F1 and SEC-F3 which may refer to bigger positive charge.
5) The FT-IR spectrum of SEC-F2 fraction of BWP shows the presence of characteristic functional groups of alcohol, hydroxyl, alkane, aldehydes and alkenes groups, nitro, aromatic amines, aliphatic amines and alkyl halides compounds.
6) Buffalo whey protein tested for antibacterial activity against Staph. pasteuri, SEC-F2 of BWP gave the maximum bacterial inhibition than SEC-F1 and SEC-F3.
7) The minimum inhibitory concentration of SEC-F2 was found to be 200 µg/ml against Staph. pasteuri.
8) Evaluation of antibacterial activities of mixture of SEC-F2 with vancomycin, tetracyclin and amoxicillin has less effect on Staph. pasteuri than SEC-F2 without antibiotics.
9) TEM images of SEC-F2 treated bacteria show various signs of cellular deformation, indicating on direct disruptive action of SEC-F2 on the cell wall and cell membrane.
10) In the present study leaves and seeds of M. oleifera were collected from Zagazig, identified and with successive extraction method with hexane, ethanol and water extracts were screened for their antibacterial. Ethanolic extract of leaves and seeds of M. oleifera were screened against the tested identified bacteria.
11) Antibacterial activity of these extracts against Staph. pasteuri showed that ethanolic extract of moringa leaves and seeds has the most effective extract.
12) The minimum inhibitory concentration of moringa leaves ethanolic extract was found to be 50 µg/ml and seeds ethanolic extract was found to be 400 µg/ml against Staph. pasteuri.
13) Quantitative inhibition of Staph. pasteuri at O.D.600by ethanolic extract of moringa leaves and seeds showed inhibition in bacterial growth compared to control.
14) In this study moringa leaves and seeds inhibit Staph. pasteuri at lowest concentration 50g/ml for leaves and 400 g/ml for seeds. A mixture of aqueous ethanolic moringa leaves and seeds extract with vancomycin, tetracycline and amoxicillin has less effect on Staph. pasteuri than leaves and seeds without antibiotics.
15) Detection and purification of antibacterial compounds by paper chromatography to separate active substances from moringa leaves and seeds, these substances detected by FT-IR, UV and GC-Mass. FT-IR for leaves and seeds showed that leaves and seeds contains functional groups of alcohol, hydroxyl, alkane, aldehydes and alkenes groups, aromatic amines, aliphatic amines and alkyl halides compounds, and UV for leaves and seeds showed a peak centered near to 300nm.and GC-Mass for leaves showed seven principle peaks that correspond to 19 active compounds and 11 active compounds for seeds. This showed that the most active compounds has antimicrobial activity are alcoholic, olefene, ketonic derivatives, esters and fatty esters compounds.
16) TEM images of moringa seeds and leaves against Staph. pasteuri show various signs of cellular deformation, indicating on direct disruptive action of moringa seeds and leaves on the cell wall and cell membrane.
17) Estimation of total phenolic, flavenoids and antioxidants compounds of moringa leaves and seeds ethanolic extract showed the highest level content of phenolic, flavenoids and antioxidants compared to control.
18) AgNPs derived from moringa leaves ethanolic extract and SEC- of BWP has less activity against Staph. pasteuri compared to moringa and SEC-F2 as raw material without nanoparticles.
The fast growing of microorganisms resistant to multi antimicrobial drugs have vast impact on mortality and human healthcare. The antimicrobial peptides are highly active against most microbes such as Gram-positive and Gram-negative bacteria, fungi, viruses by disruption of cell membrane. The plants have been used for medicinal purpose as old as the history of mankind. Thus extraction and characterization of several active phytocompounds from the medicinal plants is the basis for the formation of some high activity profile drugs. Further, the phytochemical contents and pharmacological actions. Moringa plants having medicinal potential remain UN assessed by rigorous scientific research to define efficacy and safety. The leaves, fruits, flowers and immature pods of this tree are used as a highly nutritive vegetable in many countries. M. oleifera leaves has been reported to be a rich source of -carotene, protein, vitamin C, calcium and potassium and act as a good source of natural antioxidants.
1) one hundred isolates collected from different patients suffered from different diseases from ZUH, and from these isolates only 32 isolates showed blood hemolysis, these 32 isolates experimented against buffalo whey protein and moringa leaves and seeds extracts and the most sensitive isolates identified by biochemical testes and molecular identification by 16SrRNA which identified as Staph. pasteuri MN368257.
2) In this study buffalo whey protein was hydrolyzed with papain, trypsin and pepsin enzymes to liberate the antibacterial peptides. The extent of protein degradation by trypsin was estimated by assessing the degree of hydrolysis (DH) and SDS-PAGE analysis. The hydrolysate obtained after 180 min. degradation had the highest DH (23%) in comparison with those obtained after 60 and 120 min (9% and 18%, respectively).
3) Molecular mass of BWP as obtained by SDS-PAGE as affected by trypsin at different times 0,1,2 and 3hr. showed two major peaks Size exclusion chromatography fraction (SEC-F1, SEC-F2 and SEC-F3) at 2hr., peaks were collected, lyophilized and assessed for their antibacterial activity.
4) Molecular mass of three fraction of BWP as obtained by UREA-PAGE, the faster migration of SEC-F2 on urea-page into cathode direction than hydrolyzed protein, SEC-F1 and SEC-F3 which may refer to bigger positive charge.
5) The FT-IR spectrum of SEC-F2 fraction of BWP shows the presence of characteristic functional groups of alcohol, hydroxyl, alkane, aldehydes and alkenes groups, nitro, aromatic amines, aliphatic amines and alkyl halides compounds.
6) Buffalo whey protein tested for antibacterial activity against Staph. pasteuri, SEC-F2 of BWP gave the maximum bacterial inhibition than SEC-F1 and SEC-F3.
7) The minimum inhibitory concentration of SEC-F2 was found to be 200 µg/ml against Staph. pasteuri.
8) Evaluation of antibacterial activities of mixture of SEC-F2 with vancomycin, tetracyclin and amoxicillin has less effect on Staph. pasteuri than SEC-F2 without antibiotics.
9) TEM images of SEC-F2 treated bacteria show various signs of cellular deformation, indicating on direct disruptive action of SEC-F2 on the cell wall and cell membrane.
10) In the present study leaves and seeds of M. oleifera were collected from Zagazig, identified and with successive extraction method with hexane, ethanol and water extracts were screened for their antibacterial. Ethanolic extract of leaves and seeds of M. oleifera were screened against the tested identified bacteria.
11) Antibacterial activity of these extracts against Staph. pasteuri showed that ethanolic extract of moringa leaves and seeds has the most effective extract.
12) The minimum inhibitory concentration of moringa leaves ethanolic extract was found to be 50 µg/ml and seeds ethanolic extract was found to be 400 µg/ml against Staph. pasteuri.
13) Quantitative inhibition of Staph. pasteuri at O.D.600by ethanolic extract of moringa leaves and seeds showed inhibition in bacterial growth compared to control.
14) In this study moringa leaves and seeds inhibit Staph. pasteuri at lowest concentration 50g/ml for leaves and 400 g/ml for seeds. A mixture of aqueous ethanolic moringa leaves and seeds extract with vancomycin, tetracycline and amoxicillin has less effect on Staph. pasteuri than leaves and seeds without antibiotics.
15) Detection and purification of antibacterial compounds by paper chromatography to separate active substances from moringa leaves and seeds, these substances detected by FT-IR, UV and GC-Mass. FT-IR for leaves and seeds showed that leaves and seeds contains functional groups of alcohol, hydroxyl, alkane, aldehydes and alkenes groups, aromatic amines, aliphatic amines and alkyl halides compounds, and UV for leaves and seeds showed a peak centered near to 300nm.and GC-Mass for leaves showed seven principle peaks that correspond to 19 active compounds and 11 active compounds for seeds. This showed that the most active compounds has antimicrobial activity are alcoholic, olefene, ketonic derivatives, esters and fatty esters compounds.
16) TEM images of moringa seeds and leaves against Staph. pasteuri show various signs of cellular deformation, indicating on direct disruptive action of moringa seeds and leaves on the cell wall and cell membrane.
17) Estimation of total phenolic, flavenoids and antioxidants compounds of moringa leaves and seeds ethanolic extract showed the highest level content of phenolic, flavenoids and antioxidants compared to control.
18) AgNPs derived from moringa leaves ethanolic extract and SEC- of BWP has less activity against Staph. pasteuri compared to moringa and SEC-F2 as raw material without nanoparticles.