الفهرس 
REVIEW OF LITERATUREOnly 14 pages are availabe for public view
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Abstract
The Incidence Of Cancer Is Increasing Worldwide; Likewise, The Emergence Of Antibiotic-Resistant Biofilm-Forming Pathogens Has Led To A Tremendous Increase In Morbidity And Mortality. This Study Aimed To Evaluate The Probiotic Properties Of Bacteriocin-Producing Enterococcus Sp. With A Focus On Their Anti-Biofilm And Anticancer Activities. Three Of 79 Enterococcus Isolates (FM43, FM65, FM50) Were Identified As Producers Of Broad-Spectrum Bioactive Molecules And Were Molecularly characterized As Enterococcus Faecium By 16S Rrna Sequencing. Phenotypic And Genotypic Screening For Potential Virulence Factors Revealed No Factors Known To Promote Pathogenicity. Treatment With Proteinase K Resulted In Diminished Antimicrobial Activity; PCR-Based Screening For Bacteriocin Genes Suggested The Presence Of Both Enta And Entb Genes That Encode Enterocins A And B, Respectively. Maximum Antimicrobial Activity Was Detected During The Early Stationary Phase, While Activity Disappeared After 24 H In Culture. Bacteriocins from These Isolates Were Stable At High Temperatures And Over A Wide Range Of Ph. Interestingly, Crude Supernatants Of E. Faecium FM43 And E. Faecium FM50 Resulted In Significant Destruction (80% And 48%, Respectively; P < 0.05) Of Streptococcus Mutans (S. Mutans) ATCC 25175-Associated Preformed Biofilms. Moreover, In Vitro Cytotoxicity Assays Revealed That Extracts from E. Faecium Isolates FM43, FM65 And FM50 Inhibited Caco-2 Cell Proliferation By 76.9%, 70%, And 85.3%, Respectively. Taken Together, The Multifunctional Capabilities Of The Microbial-Derived Proteins Identified In Our Study Suggest Potentially Important Roles As Alternative Treatments For Biofilm-Associated Infections.