الفهرس 
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Abstract
HCC is one of the most dangerous complications of liver cirrhosis. The incidence of HCC in patients with HCV cirrhosis is high. Therefore, genetic variations play an important role in development of HCC especially in cirrhotic HCV patients.
A functional polymorphism at position 61 has described with G/G or A/G genotypes. a positive association between EGF 61A > G polymorphism and risk of HCC among cirrhotic HCV patients. the EGF 61*G allele is highly associated with the increased risk of HCC compared with the A allele, where; cirrhotic patients with G/G and A/G genotype had a high rate for developing HCC, respectively when compared with A/A genotype patients.
The present study was held to study the association between epidermal growth factor gene polymorphism and development of HCC in cirrhotic HCV patients.
The study was conducted on 90 subjects in Alexandria Main University Hospital, Tropical Medicine Department, the subjects were divided into three groups. The first two groups consisted of 60 patients each. group I contained patients with cirrhotic HCV patients with HCC while group II contained cirrhotic HCV patients without HCC. group III consisted of 30 healthy controls.
Patients were excluded from the study if they had other causes of liver cirrhosis as HBV, autoimmune hepatitis, NASH and suspected malignancies other than HCC.
All patients and controls were subjected to the following:
1) Detailed history taking and clinical examination.
2) Routine laboratory investigation:
a) Complete blood picture.
b) Liver function tests:
AST, ALT.
Serum bilirubin (total, direct).
PT, INR.
Serum albumin.
Alkaline phosphatase.
c) Renal function tests: serum urea and serum creatinine.
Fasting blood sugar.
3) HBs Ag, HBcore Ab,HCVAb and PCR for HCV.
Serum AFP determination. DNA extraction (Qiagen DNA extraction kit) and genotyping assay of EGF+61/G polymorphism (Taqmangenotypin master mix) using the fluorogenic 5’-nuclease TaqMan Real time PCR assay