الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Acute myeloid leukemia (AML) consists of a group of clonal malignant diseases characterized by a deregulated proliferation of immature myeloid cells, enhanced self-renewal, decreased cell death, genomic instability, dissemination and uncontrolled proliferation/ survival of immature myeloid progenitors that undergo a differential block at may maturation steps leading to the accumulation of leukemic cells in the bone marrow and inhibition of normal hematopoiesis. Phosphatase and tensin homolog (PTEN) is a tumor suppressor gene that is often deleted or inactivated in many tumor types, including glioblastoma, endometrial carcinoma and lymphoid malignancies regulates PI3K/AKT pathway involved in angiogenesis, metabolism, growth, proliferation, survival, protein synthesis, and transcription.
Aim of this study was to determine the levels of gene expression of PTEN gene in patients with acute myeloid leukemia.
The studied group included 30 patients diagnosed as de novo AML as group A and 30 health subjects as control group B matching for age and sex. Thorough history, physical examination and routine investigations were done. Real time PCR for PTEN gene expression where PCRs was performed using primers and probes of PTEN with an ABI Prism GeneAmp 7500 using peripheral blood.