الفهرس 
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Abstract
Impact of abiotic elicitors (poly ethylene glycol (PEG), NaCl and UV-C) and alkaloids precursors, arginine (Arg) and ornithine (Orn) on callus growth and biochemical parameters of Egyptian henbane was studied. Results cleared that, the highest values of fresh (FW) and dry (DW) weight of callus attributed with high activity of peroxidase (POD) and superoxide dismutase (SOD) as well as high concentration of anthocyanin were detected in 10 dS/m of NaCl-stressed callus after 21d of explanting. Although 2.5 % of PEG stressed callus had the highest concentration of carbohydrates, 5% of PEG stressed-one gave the highest amount of Chl.a and b, amino acids, protein, total alkaloids, hyoscyamine as well as high activity of POD. Also, PEG at 10 % gave the highest amount of Chl.a, carotenoids, proline, malondialdehyde (MDA) and hyoscyamine. Although callus exposed to 1h of UV-C synthesized the highest content of free phenolics but calli exposed to 2h of UV-C had the highest concentration of glutathione. Unstressed callus had the highest amount of H2O2 and the highest activity of catalase (CAT). However, both Orn or Arg increased the FW, DW, Chl.a, b and total carbohydrates in callus after 30d of explanting. Low level of Orn increased the concentration of total alkaloids, hyoscyamine, glutathione and decreased level of H2O2. High level of Arg increased the free phenolics and activity of POD. Effect of different abiotic stressors and alkaloid precursors on histology and protein profile was also discussed. It can be concluded that, PEG stressed–callus was powerful technique for alkaloids enhancement and it must be re-evaluate on large scale production.