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العنوان
Microbiological Study on Pathogenic Yersinia Spp Isolated from Clinical Samples from Abu Rish Hospital of Children
Cairo University, Egypt /
المؤلف
Alsaadawi, Tarek Farouk Hanfi.
هيئة الاعداد
باحث / Tarek Farouk Hanfi Alsaadawi
مشرف / Doaa Mohamed Abdelaty Ghaith
مشرف / Mahmoud M.M. Zaky
مشرف / Omneia Fekry Hassan
مناقش / Mohammed Ismail Abu Dubara
مناقش / Amr Said Hanoura
تاريخ النشر
2021.
عدد الصفحات
147 p. ;
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Multidisciplinary تعددية التخصصات
تاريخ الإجازة
10/3/2021
مكان الإجازة
جامعة بورسعيد - كلية العلوم ببورسعيد - botany
الفهرس
Only 14 pages are availabe for public view

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Abstract

Gastrointestinal disorders refer either to acute enterocolitis, particularly in children, or to pseudo-appendicitis, in young adults In this study, pathogenic strains of Y. enterocolitica were identified by microbiological and PCR methods. The samples were collected from stool samples of children suffering from acute diarrhea. The techniques were used to isolate Y. enterocolitica from the samples PSB, using CIN medium, biochemical and PCR methods. PCR methods based on the identification of YadA virulence gene & 16S rRNA for detection, were used for the specific detection of the pathogenic strains of Y. enterocolitica. DNA Sequencing of 16S rRNA gene was conducted in both directions and a consensus sequence of 1476 bp was used for nucleotide (nt.) analysis. Partial DNA sequences were submitted to GenBank database and obtained accession numbers; MK168055 and MK168056 for Y.Ent-1/EGY018 and Y.Ent-2/EGY018 strains, respectively. Identification of homologies between nucleotide sequence of the studied Yersinia Spp. isolates and others published in GenBank was done using BLAST 2.2 search program (National Center for Biotechnology Information “NCBI” http://www.ncbi.nlm.nih.gov/). Both Y.Ent-1-EGY2018 and Y.Ent-2-EGY2018 strains revealed high similarity (96.6%) between each other. Sequence analysis of Y.Ent-1- EGY2018 and Y.Ent-2-EGY2018 showed 96.3-97.6% and 97.1-98.5% identity (respectively) when compared to other available Yersinia enterocolitica partial sequences of 16S rRNA gene published in GenBank. Phylogenic analysis of 16S rRNA gene partial sequence showed that Y.Ent-2-EGY2018 strain was isolating in same cluster of Yersinia enterocolitica but exists in different branch. However, Y.Ent-2-EGY2018 strain is present alone in a separate cluster.