الفهرس 
DECLARATIONOnly 14 pages are availabe for public view
 |  | from | 117 |  |  |
| | | from | 117 | | |
Abstract
The present study aimed to valorize the protein-rich wastes for the production of protease enzyme (one of the most important industrial enzyme) by bacteria candidates isolated from the environment. In this regard, this study included 5 pats as follows:
1. Isolation and Screening of Protease Producing Bacteria.
2. Identification of the Most Potent Bacterial Isolates.
3. Optimization of Protease Production.
4. Partial characterization of the Crude Protease.
5. Hydrolysis of Protein-Rich Wastes by the Crude Protease.
The obtained results can be summarized as follows:
1. Isolation and Screening of Protease Producing Bacteria
Protein-rich wastes were chosen as suitable sources for isolation of protease producing bacteria. Seven bacterial isolates were isolated using nutrient agar. These isolates were subjected to primary screening through cultivating on skimmed milk agar plates to select protease producing isolates. In order to select the most potent protease producing bacterial isolates, the isolates that showed considerable clear zone of hydrolysis on skimmed milk agar plates were selected and subjected to secondary screening using minimal liquid medium supplemented with casein as a sole carbon source. Results of secondary screening revealed that the highest significant levels of protease activity (P<0.05) were (0.124 ± 0.006 and 0.050 ± 0.001 pmol/ml/min) from the isolates No. 1 and 3, respectively. These isolates were selected for further experiments.