الفهرس 
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Abstract
BC is a heterogeneous disease with diverse histological patterns and biological features which results in distinct clinical behaviors and is the most common invasive cancer in females worldwide. It accounts for 16% of all female cancers and 22.9% of invasive cancers in women.
DNA methylation is an important determinant of genomic stability and gene expression. C677T polymorphism decrease MTHFR enzyme activity that results in DNA hypomethylation. Also elevated S’adenosyl homocysteine caused by MTHFR deficiency inhibits DNA methyl transferases activity, resulting in DNA hypomethylation. Resultant hypomethylation increases proto-oncogenes expression, which could explain the association between this polymorphism and some types of cancer such as breast cancer.
The aim of this study was to assess the relationship between MTHFR gene polymorphism and breast cancer in Egyptian patients.
This study was conducted on 100 women their ages ranged between 30-82 years. They were divided into 2groups: group I (breast cancer) included 50 women, their age ranged between 30-80 years. group II (Control Group) included 50 ages matched apparently healthy women their ages ranged between 30-72 years.
MTHFR gene C677T single nucleotide polymorphism analysis was done by real time PCR.
Genotype distribution of MTHFR: TT genotype and T allele were statistically significant higher in BC group than in control group, marital status (single) and increase CA 15.3 value were significantly higher in TT group than both of CC and CT groups.
Univariate logistic regression model for the risk factors of BC revealed that decrease in HB, increase in AST, CA 15.3 values, and prescence of CT and TT were risk factors for BC, multivariate logistic regression model revealed that decrease in HB, increase in CA 15.3 values and presence of TT genotypes were dependent risk factor for BC.