الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Chronic lymphocytic leukemia (CLL) is defined as a malignant lymphoproliferative disorder of small, mature-appearing monoclonal B-lymphocytes in the blood cells, bone marrow and lymph nodes. In Egypt, CLL was the most common subtype of leukemias, affecting primarily the elderly men, with the mean age of presentation being 59 year.
The onset of CLL is insidious about 25-50% of CLL patients are asymptomatic at the time of presentation, and it is usual for CLL to be discovered incidentally after a blood cell count is performed for another reason.
A definitive diagnosis of CLL is based on the combination of a lymphocytosis and characteristic lymphocyte morphology and immunophenotype. The CLL scoring system is based on the presence or absence of five markers (SmIg, CD5, CD23, CD22/CD79B, FMC7)
MiRNAs are recently identified class of small single stranded RNA molecules of 21-23 nucleotides that regulate gene expression at the posttranscriptional level. It has been shown that miRNAs regulate critical biological processes such as development, differentiation, hematopoiesis, metabolism, cell cycle and apoptosis.
Multiple recent studies showed that genetic polymorphisms play an influential role in CLL susceptibility, treatment, response and prognosis. One of the important polymorphisms is miR 196a2. The SNP in mir-196a2 rs11614913 C/T, have been implicated as possible biomarker associated with multiple kind of cancers as non–small cell lung cancer (NSCLC) and epithelial ovarian cancer.
This study was done to determine SNP in miR 196a2 rs11614913 with RQ-PCR using TaqMan SNP genotyping assay. The study was carried out on 80 subjects divided into two equal groups. The first group included 40 newly diagnosed CLL patients admitted to Alexandria University Main Hospital. The second group of 40 healthy subjects as a control group with no history of malignant neoplasm.
All patients in the study were assessed by detailed history taking, complete clinical examination and routine laboratory investigations as well as immunophenotypying to diagnose CLL. DNA was extracted by (QIAamp blood Mini kit QIAGEN) from peripheral blood samples. Purified DNA was quantified using Nano DROP 2000 Spectrophotometer. Then the purified DNA was genotyped with RQ-PCR system using TaqMan SNP genotyping assay on Rotor gene Q.