الفهرس 
• ThalassemiaOnly 14 pages are availabe for public view
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Abstract
ABSTRACT
Corresponding author: Marwa Mostafa Mohamed
Mobile no.:01006466358 e-mail: marwa.mostafa@med.asu.edu.eg
Background: As the life expectancy of β-thalassemia patients increases, new complications are becoming more recognized. Many recent studies have associated thromboembolic complications with β-thalassemia. It was shown that the hypercoagulable state in β-thalassemia is multifactorial, with much evidence pointing to the contribution of submicrometric vesicles, released from different cell types by budding, and accused of being mediators of inflammation and thrombosis. These vesicles are termed “Microparticles” (MPs).
Aim of work: To determine the amount of total and cell type specific MPs in plasma of patients with β-Thalassemia major (β-TM) and Thalassemia intermedia (TI), in comparison to a control group, and to evaluate the relationship between the amount of MPs and thromboembolic complications, blood transfusion and iron chelation therapy. It also aimed to study the expression of anionic phospholipids on the red blood cells (RBCs) of β-thalassemia patients.
Patients and Methods: The current study was carried out on 45 thalassemic patients; 30 patients with β-TM and 15 with 15 β-TI, attending regular follow up visits at the Pediatric Hematology Clinic, Pediatric Hospital, Ain Shams University. Ten control subjects were included. A flow cytometer (FCM) was used to detect total and cell type specific MPs.
Results:
Total absolute MPs, platelet MPs (PMPs) (absolute value) and PS+PMPs (percentages and absolute values), were significantly higher among the patients’ group collectively and among each of the thalassemic subgroups individually (β-TM or β-TI), when compared to the control group. Study of diagnostic performance and ROC curve analysis of plasma MPs assigned (2190 /µl) as the best cutoff value for discriminating between thalassemic patients and control subjects with an 89% sensitivity and 100% specificity.
Conclusion: total MPs and PMPs levels in plasma of thalassemic patients are higher than normal subjects. Elevated total MPs and PMPs reflect a state of cellular stress and alteration, and may be contributing in the process of thrombosis taking place, either silently or overtly in β-thalassemia. With further studies and standardization of MPs measurement and quantification, total MPs and PMPs could be useful biomarkers for hypercoagulability in thalassemia