الفهرس 
Acknowledgements
Hypertensive Disorders of PregnancyOnly 14 pages are availabe for public view
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Abstract
This study was conducted on 80 pregnant women in second trimester with age ranged from 18 to 40 years old, 40 pregnant women with high risk pregnancy for preeclampsia and 40 pregnant women with high insulin resistance. All participants were recruited from Obstetrics and Gynecology Department, Women’s Health Hospital, Assiut University. Practical work was carried out at Clinical Pathology Department, Assiut University Hospital. All individuals were subjected to the following: I- Personal history as (age, occupation, resident). II- Obstetric history as (parity, gestational age). III- Ask about risk factor of preeclampsia as: primigravida, diabetes mellitus, kidney disease, chronic hypertension, previous or family history of preeclampsia, multiple gestation. IV- Clinical examination: cardiac and chest examination, blood pressure measurement, body mass index. V-Laboratory investigations: Routine laboratory investigations: CBCFasting serum glucoseKidney function tests Liver function testsSpecial laboratory investigations: Serum fasting insulin level by chemiluminescence immunoassay and calculate HOMA-IR= Fasting insulin(mU/L) × fasting glucose(mmol/l) 22.5Plasma miR-210 by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The current study showed the following findings: In the present study, 8 pregnant women out of 40 cases of high risk group developed PE and 6 pregnant women out of 40 cases of high insulin resistance developed PE. - Plasma miR-210 was significantly higher in high insulin resistance group compared to risk factors group, with p-value 0.004. - Plasma MicroRNA 210 was significantly higher in pregnant women with PE compared to pregnant women without PE in both groups (risk factors group and high insulin resistance group), with p-value 0.001 and 0.003 respectively. -Plasma MicroRNA 210 was significantly higher in high insulin resistance group without preeclampsia (NO.=34) compared to risk factors group without preeclampsia (NO.=32), with p-value <0.001. In high IR group, there was statistically significant weak positive correlation between miR-210 and fasting insulin (r =0.314, p =0.049). Also, there was weak positive correlation between miR-210 and HOMAIR (r=0.301) and there was tendency to be significant with (p=0.05). - We assessed the predictive value of plasma miR-210 in women with risk factors who developed PE versus non-preeclampsia. The results show that at a cut-off value =2.28 fold change, plasma miR-210 was 87.5 % sensitive and 68.8% specific for diagnosis of PE vs. non-preeclampsia in risk factor group, with AUC of 0.852. Plasma miR-210 was significantly higher in high risk group with PE compared to those without PE (p= 0.001). - We assessed the predictive value of plasma miR-210 in high IR with PE vs. non-PE. The results show that at a cut-off value= 9.34 fold change, plasma miR-210 was 83.3% sensitive and 68.8% specific for diagnosis of PE vs. non-PE in high IR group, with area under the curve of 0.868. Plasma miR-210 was significantly higher in high IR group with PE compared to high IR group without PE (p=0.003).