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العنوان
Detection of Mutations Polymorphism in Connexin 26 and ribosomal RNA in vertebrates/
المؤلف
El-Sherif, Ghada Mohammed Abd-ElWareth
هيئة الاعداد
باحث / غاده محمد عبدالوارث الشريف
مشرف / صبحى السيد حسب النبى
مشرف / خالد محمد عبدالله جبه
مشرف / أسماء صلاح مختار معطى
الموضوع
Phylogenetic Analysis PCR Amplification of Connexin 26 gene
تاريخ النشر
2020
عدد الصفحات
76 P.:
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الحيوان والطب البيطري
تاريخ الإجازة
1/1/2020
مكان الإجازة
جامعة المنوفية - كلية العلوم - علم الحيوان
الفهرس
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Abstract

Family Lethrinidae (Sho´our fishes, Shaari fishes) is one of the key families for Red Sea and Indian Ocean fisheries. Some of these species Were recorded in the IUCN Red List of species as endangered. In order to develop simple genetic marker that aid their rapid identification, fin clips of three lethrinid species were collected from local fish markets in Hurghada and Suez cities. These species were Lethrinus lentjan, L. harak, L. microdon, L. mahsena. DNA was extracted from these samples and subjected to PCR amplification for the mitochondrial 12 srRNA nuclear and the nuclear 5srRNA mitochondrial 12 srRNA genes. The 12srRNA PCR amplicons were sequenced. GenBank comparison and phylogenetic analysis for the 12srRNA gene sequences resulted in clear identify for the sampled species with L.lentjan, L. harak, L. microdon and L. mahsena. Meanwhile, a simple, 2 % agarose gel electrophoresis for the 5srRNA ran in a low voltage for 2 hours the amplicons were resolved in a simple, 2 % agarose gel run at low voltage for 2 hours.