الفهرس | Only 14 pages are availabe for public view |
Abstract Family Lethrinidae (Sho´our fishes, Shaari fishes) is one of the key families for Red Sea and Indian Ocean fisheries. Some of these species Were recorded in the IUCN Red List of species as endangered. In order to develop simple genetic marker that aid their rapid identification, fin clips of three lethrinid species were collected from local fish markets in Hurghada and Suez cities. These species were Lethrinus lentjan, L. harak, L. microdon, L. mahsena. DNA was extracted from these samples and subjected to PCR amplification for the mitochondrial 12 srRNA nuclear and the nuclear 5srRNA mitochondrial 12 srRNA genes. The 12srRNA PCR amplicons were sequenced. GenBank comparison and phylogenetic analysis for the 12srRNA gene sequences resulted in clear identify for the sampled species with L.lentjan, L. harak, L. microdon and L. mahsena. Meanwhile, a simple, 2 % agarose gel electrophoresis for the 5srRNA ran in a low voltage for 2 hours the amplicons were resolved in a simple, 2 % agarose gel run at low voltage for 2 hours. |