الفهرس 
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Abstract
Background: Delayed healing of diabetic wounds has been well-documented. Recently, the concept of platelet-rich plasma (PRP) treatment to utilize a patient’s own platelets and growth factors to improve healing at the site of injury has been used extensively in many medical fields.
The aim of this study was to evaluate the effect of PRP on wound healing in the tongue of normal and induced diabetic albino rats.
Materials and Methods: A total number of 144 adult male albino rats (average weight = 200gm) were used and were classified into two main groups: non-diabetic group and Streptozotocin-induced diabetic group. Each group was further divided into three subgroups: non-treated wound, PRP-treatment before wound, and PRP-treatment after wound. The incisional wound was made on the lateral border of the rat tongue.
Tongue specimens were dissected on postoperative days 1, 3, 7 and 10 and examined histologically by H&E and Masson’s trichrome stain, immunohistochemically by p63 and vimentin, and ultra-structurally by TEM. Image analysis was performed in which p63 and VIM immunoreactivity was measured as area fraction and then statistical analysis was performed by using ANOVA test and multiple linear regression analysis.
Results:
H&E Stain Results: The most accelerated re-epithelialization was revealed in the subgroups treated with PRP before the wound.
Masson’s trichrome Stain Results: The wound areas showed blue stained collagen fibers with ranging thickness and arrangement in the lamina propria; with presence of granulation tissue filling wound gap and newly formed blood vessels which were more abundant in PRP-treated wounds.
p63 Immunohistochemical Results: p63 positive nuclear immune reaction in the wound margins and healed epithelium was shown, ranging in intensity and in distribution.
Vimentin Immunohistochemical Results: Granulation tissue was shown filling the wound gap with intense VIM positive nuclear immune reaction of inflammatory cells; and VIM positive nuclear immune reaction of fibroblasts and endothelial cells of newly formed blood vessels were shown in the lamina propria.
Image Analysis Results: The peak mean value of p63 area fraction was observed in the non-diabetic subgroup in the 1st day postoperative which was treated with PRP after the wound. While the peak mean value of VIM area fraction was observed in the diabetic subgroup in the 3rd day postoperative which was treated with PRP before the wound.
TEM Results: Enhanced wound healing was revealed in the PRP-treated subgroups. Defensive cells were present in the lamina propria in the PRP-treated subgroups. Vacuoles and bacterial organisms were displayed in diabetic non-treated subgroups.
Conclusion: PRP could be used to prevent expected impaired wound healing in diabetic oral mucosal wounds and to accelerate wound healing in non-diabetic oral mucosal wounds.
Recommendation: It is recommended to use a single PRP injection as a prophylactic when dealing with diabetic and non-diabetic oral mucosal wounds. More research is required to further study the effect and quantity of each growth factor contained in PRP.