الفهرس 
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Abstract
Delayed socket healing is the most frequent side effect of
methylprednisolone, therefore the present study was carried out to
investigate the topical effect of using Aloe Vera gel versus Silver
Nanoparticles gel on socket healing in MP treated Albino Rats using
histological and SEM studies.
This study was carried out on 32 adult male Albino rats and they were
divided into three groups.
- group I (Control group)
1) Subgroup A: they received IM injection of sterile normal saline (0.2
mg/kg, 3 times/week for 5 weeks). After this period, lower right first
molars were extracted.
2) Subgroup B: they received IM injection of methylprednisolone acetate
(0.2 mg/kg, 3 times/week for 5 weeks). After this period, lower right first
molars were extracted.
- group II as group IB and the sockets were filled with 92% Aloe Vera
gel immediately after extraction.
- group III as group IB and the sockets were filled with AgNps gel
immediately after extraction.
All rats of all groups were anaesthetized then the lower right first molars were extracted. The sockets of group I (subgroup A, B) were sutured
then left to heal by themselves without any treatment. The sockets of group
II were dropped with 92% Aloe Vera gel and AgNps gel in group III. Then
the sockets were sutured to stabilize the gel in the socket. Some rats from
group I were sacrificed by cervical decapitation at day zero, just after
extraction. The rest of the rats from group I, II and III were sacrificed by
cervical decapitation at day 7 and day 14.
After sacrification the mandible was dissected out, the right half of the
mandible containing the socket was processed for subsequent light
microscopic examination using Haematoxylin and Eosin stain and scanning
electron microscopic examination.
The LM specimens were fixed in 10% buffered formalin and
decalcified in 10% neutral-buffered EDTA. Then they were processed for
routine H&E stain in order to assess the healing of the socket in different
groups. While the SEM specimens were fixed in 2.5% glutaraldehyde in 1M
phosphate buffer. Then processed for SEM examination. Histomorphometic
measurement using image j software was done to measure the percentage of
new bone formation in the extraction socket in different groups and at
different observation periods. Moreover, the number of bone cells
(osteoblasts, osteocytes and osteoclasts) was counted by image j program in different groups and at different observation periods. Then the data was analyzed using the SPSS (version 22) data processing software to compare
between the groups using the ANOVA test followed by tukey’s post hic test
and t-test.