الفهرس 
Anticancer ScreeningOnly 14 pages are availabe for public view
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Abstract
A series of imidazolin‐2‐thione derivatives was synthesized and structurally confirmed through
the use of different spectroscopic techniques such as infrared, nuclear magnetic resonance, and
mass spectrometry along with elemental analyses. The breast cancer cell line MCF‐7 was
utilized in the evaluation of the cytotoxic activity of the prepared molecules The tested
molecules 3. and 7 exhibited the best results on MCF‐7 cells, with mean IC50 values of 3.26
and 4.31 μM, respectively. The results of the VEGFR‐2 assay indicated that compounds 3 and
7 displayed a good inhibition of the VEGFR‐2 kinase enzyme. Additionally, DNA flow
cytometry of compounds 3 and 7 showed cell cycle arrest at the G0/G1 phase, cell apoptosis,
and marked DNA fragmentation in MCF‐7 cells. Finally , compounds 3 and 7 were proved to
up regulate the activation of effector caspase‐3/7, as presented by the caspase‐3/7 green flow
cytometry assay .