الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 95 |  |  |
| | | from | 95 | | |
Abstract
Aim This study was planned to detect the effect of Chlorella vulgaris powder on the growth performance, some hematological parameters, immune system and the ability of this algae to protect Tilapia fish from the adverse effects of lead. Materials and Methods This work was carried out on 180 Oreochromis niloticus fish with an average weight of 60-65gm which were divided into 3 experiments total 9groups (each group of 20 fish). Experiment (1) includes G1and G2 to detect the effect of Chlorella vulgaris 5% as growth promoter with special reference to its effect on some hematological and biochemical parameters.G1 (control group). G2 (Chlorella group) which fed on diet contain 5% Chlorella vulgaris for 30 days. Experiment (2) includes G3, G4 and G5 to detect the effect of Chlorella vulgaris 5% as immunmodulator. G3fish take Chlorella for 2 weeks as a protection then injected I/P with dexamethasone 0.2 mg / fish for one week as immune suppressive agent. G4fish injected with dexamethasone 0.2 mg/ fish for one week then take Chlorella in diet as a treatment for 2 weeks. G5fish take basal diet along dexamethasone exposure period (one week).Experiment (3) includes G6, G7, G8 and G9 to detect the ability of Chlorella vulgaris 5% to decrease the adverse effect of lead on fishG6fish take Chlorella for 2 weeks as a protection then exposed to lead nitrate 2.5 mg/ liter of water for two weeks.G7Fish first exposed to lead nitrate 2.5 mg/ liter of water for two weeks then treated with Chlorella for 2 weeks.G8fish feed on Chlorella along lead exposure period (2 weeks). G9 fish fed on basal diet along lead exposure period (2weeks).Blood samples were collected at the end of experiment for hematological and biochemical parameters. Tissue specimens from liver, kidney, gills and muscle were collected from experiment(3)for histopathological examination Results The results of experiment1 showed that There is a significant increase in FBW, BWG, DWG, RWG and SGR in Chlorella group. There is a significant increase in total protein, albumin and globulin in Chlorella group. The total cholesterol, triglyceride and LDL higher in control group but HDL showed a significant increase in Chlorella group. For Experiment2There is a significant increase in RBCs, Hb, PCV, WBCs and IgM levels in all groups fed on Chlorella. For experiment3 Chlorella vulgaris able to decrease the adverse effects of lead on vital organs (liver and kidney) and blood parameters. For hematology it was found that the lead nitrate causes a significant decrease in hematological but 5% chlorella vulgaris microalgae resulted in a significant increase in this parameters. For liver and kidney functions the lead nitrate caused a significance increase in ALT, AST, creatinine and uric acid levels in serum of treated fish. Chlorella decreases the level of these parameters. The histopathological picture of affected organs was lower in groups treated with lead nitrate and fed on Chlorella vulgaris. Conclusion It could concluded that Chlorella vulgaris powder is highly valuable in fish farms as feed additives. It can be used as growth promoter in addition to its Immunostimulant effect and its ability to chelate heavy metals.