الفهرس 
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Abstract
A total of 252 samples were collected from different organs of diseased and freshly dead broiler chickens and ducks from different poultry farms at Ismailia Governorate. E.coli isolation rate was 80.6% (203/252). Thirty six of E.coli isolates were serogrouped into: O63, O111, O125, O143, O152, O157:H7, O164, O169, O55, O86a, O114, O115, O119 and O158, while 8 E.coli isolates found to be untyped. Forty E.coli isolates were examined for their antibiotic senstivity to 15 antibacterial agents (from 5 different antibiotic groups). All E.coli isolates (97.5%) were highly resistant to penicillin G and ceftriaxone, (92.5%) were resistant to cephalexin and (77.5%) were resistant to ampicillin, (62.5%) were resistant to amoxicillin/clavulanic acid and oxytetracycline, (45%) were resistant to nalidixic acid, (35%) were resistant to ciprofloxacin and neomycin, (32.5%) were resistant to norfloxacin, (22.5%) were resistant to cefotaxime and ceftazidime, while (65%) and (35%) were intermediate resistant to colistin sulphate and levofloxacin, respectively. All tested E.coli isolates were resistant to at least 3 antibiotic groups and multidrug resistance was seen. Conventional PCR (cPCR) results indicated that representative 18 E.coli isolates had virulence genes as iss, iutA and vat genes 100% (18/18), traT gene 88.8% (16/18), eaeA gene 27.7% (5/18), while only 11% (2/18) had papC gene. Also 18 E.coli isolates had betalactam and fluoroquinolone resistance genes as blaTEM, blaSHV and ampC genes100% (18/18), qnrS gene 83.3% (15/18), blaCTX-M gene 27.7% (5/18), while only 16.6% (3/18) had qepA gene.