الفهرس 
SummaryOnly 14 pages are availabe for public view
 |  | from | 136 |  |  |
| | | from | 136 | | |
Abstract
This work reported green synthesis of silver and gold nanoparticles using Pimpinella anisum seeds extract as a novel, rapid, low-cost and eco-friendly route. As a preliminary work, we screened several plant extracts and compared their potency for metal nanoparticle synthesis. P. anisum seeds extract was found to be the most potent extract towards the reduction of both Ag+ and Au3+ ions so P. anisum extract was further studied.
This work presents the first optimization study for the extraction conditions of the plant (solvent, temperature, solvent/plant ratio and extraction time), and its impact on the size, shape, and dispersity of the prepared nanoparticles. The seeds of P. anisum were extracted under various conditions and the resulted extracts were investigated for its potency in the synthesis of AgNPs and AuNPs. The prepared nanoparticles were structurally characterized by UV–vis, transmission electron microscope, dynamic light scattering and zeta potential analysis. The types of biomolecules responsible for metal ion reduction were identified using phytochemical tests and FTIR analysis. Silver and gold nanoparticles were tested for their free radical scavenging activity against 2,2-diphenyl-1-picryl-hydrazyl (DPPH), and antimicrobial activity against Escherichia coli, Staphylococcus aureus, Aspergillus flavus and Candida albicans using disk diffusion method.