الفهرس 
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Abstract
Cancer remains a major public health problem in the world with the global burden of cancer continuing to increase.Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer. HCC is the 5th most common malignancies worldwide, and is the third-leading cause of cancer-related death.Breast cancer is the most common malignancies and cause of cancer death among women in both the developing and developed world.
The aim of the present study is targeting Arginine, an oncometabolite, which plays a role in cancer proliferation by Arginase, an Arginine degrading enzyme, which in turn affects different oncogenic signals in both liver and breast cancers and might be a new approach to cancer therapy. We investigated the apoptotic mechanism and growth inhibitory effect of partially purified beef-liver Arginase in HepG2, human hepatocellular carcinoma cell line, and in both MDA-MB-231 and T-47D, human breast cancer cell lines. Enzyme purification, enzyme activity determination, WST-1 assay, morphological alteration examination, clonogenic assay, caspase-3 activity, apoptosis induction assay, QRT-PCR analysis, western blot analysis and flow cytometer analysis were performed.