الفهرس 
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Abstract
The current study aims to evaluate the role of silicon or nano silicon in controlling the chocolate spot disease of Vicia faba L. caused by Botrytis fabae. Therefore, experiments were carried out in the laboratory and greenhouse of Faculty of Agriculture, Mansoura University, Egypt. 1. Isolation, purification and identification of Botrytis fabae pathogen. Five isolates were tested and the most aggressive isolate (B1) was used. 2. Preparation of silicon and nano silicon compound. 3. characterization of nano silicon was carried out at electron microscope unit Faculty of Agriculture, Mansoura University by the help of zeta sizer instrument, EDX and scanning electron microscope that showed the purity of nano size in the nano range. 4. Green house experiments and applications of silicon and nano silicon. Giza 40 seeds were cultivated as five seeds per pot at the green house of Faculty of Agriculture, Mansoura University and watered when needed. The foliar application of Si compounds or NSi was done twice at 10 days interval (at 30th and 40th day of sowing) followed by artificial inoculation with the same spore suspension that was previously used in determining the pathogenicity test at 45th day of sowing. 5. Determination of disease severity and disease incidence. 6. Ultrastructure and physiological studies. The ultrastructure of faba plants treated with Si or NSi was carried out under TEM. Photosynthetic pigments, proline, total soluble sugars and total phenols were determined. Definsive enzymes were estimated to dtermine the significant role of silicon or nano silicon in improving plant defensive systems.