الفهرس | Only 14 pages are availabe for public view |
Abstract 1- Isolation of suspected virus through chorioallantoic membranes of embryonated chicken eggs. Detection of sheep poxvirus (SPV) by agar gel precipitation test (AGPT), indirect fluorescence antibody test (IFAT) and histopathology in field and sixth egg passaged samples. In additon, molecular characterization of SPV by conventional and real time polymerase chain reaction (PCR) and sequence analysis based on P32 gene. 2- The results indicate that SPV is existing in sheep flocks in Dakahlia Governorate, Egypt. IFAT is superior in virus antigen detection over AGPT. Sequence analysis of the amplified P32 gene of identified SPV showed close genetic relationship to Saudi Arabia virus, 2019. |