الفهرس 
Morphology and ultrastructureOnly 14 pages are availabe for public view
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Abstract
Human schistosomiasis is a tropical syndrome which can result in both acute and long-term pathological disorders. Several reports have found that PZQ, the only drug of choice, efficacy has been reduced. Modern studies are recently focusing on the antischistosomal activity of plants’ effective ingredients.
In the present study, the antischistosomal activity of the active ingredients of A. sativum (allicin, AL) and C. longa (curcumin, CU) on S. mansoni were assessed. The evaluation of both materials was applied in vitro and in vivo.
In the beginning, AL and CU have been evaluated in vitro to reflect their efficacy on different developmental stages (miracidia, cercariae, and adult worms). The miracidiacidal and cercaricidial activity of AL and CU were assayed using a series dilution of the molluscicidal LC50 (LC50, 1/2 LC50, 1/4 LC50, and 1/8 LC50). Adult worm survival was determined within three days. LC50 of AL and CU was calculated after 24 hrs of exposure to each material separately. The ultrastructure changes of the worms exposed to LC50 of AL or CU were evaluated using scanning electron microscopy. The genotoxicity of LC50 of both ingredients on S. mansoni were determined by DNA fragmentation analysis.
The in vivo study, the parasitological, physiological, histological and molecular tools were evaluated. Experimental mice were divided into non-infected and infected groups. from the week 7th post-infection (PI) all the groups begin their treatment with the different regimens. PZQ groups were treated with 300 mg/kg (P.O.) for two successive days. The AL (20, and 40 mL/kg, I.P), and CU (20 and 40 mg/kg, I.P.) were administrated for two weeks PI each alternative day.