الفهرس 
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Abstract
The present study aimed to isolate Pseudomonas aeruginosa from different clinical sources (wound, burn, urine, sputum, contact lenses, urinary catheters, and blood). Then followed by identification of P. aeruginosa using biochemical methods. Detection of biofilm producing isolates by qualitative and quantitative detection of biofilm using tube method (TM) and tissue culture plate (TCP) method and classification of different strains into strongly adherent, moderately adherent, weakly adherent and non-adherent. Afterwards, determination of antimicrobial susceptibility pattern of different antimicrobial classes according to Clinical and Laboratory Standards Institute (CLSI). PCR detection of different virulence and carbapenem resistance genes in biofilm producing isolates. Moreover, determination of different factors/agents influence biofilm formation like lysozyme and proteinase K in addition to, assessment of genetic relatedness of isolates by RAPD-PCR and ERIC PCR. Finally, statistical analysis of collected data.