الفهرس 
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Abstract
Malassezia is one of the most important yeast genera, which causes Malasseziosis in different animals. It can be transmitted to humans by contact with pets. The outcome of this work dealt with the methods of phenotypic and genotypic identification of Malassezia isolated from dogs, cats, horses, and buffaloes.
By direct microscopy using 10% KOH, fungal elements related to Malassezia spp. were observed in 32.05% (25/78) of skin scrapings from diseased (31.2%) and apparently healthy animals (33.3%). The identified Malassezia spp. were highly observed in dogs (47.82%, 11/23), followed by cats (33.3%, 5/15), horses (25%, 6/24) and buffaloes (18.75%, 3/16).
Out of 160 samples (82 ear swabs and 78 skin scrapings) from apparently healthy and diseased animals, 49 isolates; 24 from ear swabs (29.27%) and 25 from skin scrapings (32.05%) yielded positive growth on mycobiotic agar medium were suspected to be Malassezia and subjected to phenotypic and genotypic identificationmethods.
Phenotypic methods for identification of the recovered isolates includes macro and micromorphological characters as well as growth on mycobiotic agar medium at different temperatures, tween assimilation, esculin hydrolysis, tryptophan utilization, and catalase tests were used.
On mycobiotic agar medium with olive oil, lipid dependent species (M. globosa) showed creamy and rough colonies, whereas non-lipid dependent species (M. pachydermatis) on media without oil revealed raised, creamy and smooth colonies.
Concerning microscopy after Gram`s stain, M. pachydermatis were cylindrical to oval yeast cells with broad base buds, grew at 31, 37 and.