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Abstract Summary Invasive fungal infections, especially yeast infections, have recently been a serious problem in immunocompromised cancer patients which leads to high morbidity and mortality. Among Candida species, Candida albicans remains the most frequently encountered in the clinical laboratory. However; there has been an overall increase in the frequency non-Candida albicans species. Therefore, early identification of species is needed in cases of critical infections especially if accompanied by neutropenia or resistance to empirical antifungal treatment. Although the classical or conventional methods are still the standard method for identification of clinical yeast isolates, their results are time-consuming as they depend on morphological, biochemical and physiological tests. In addition, they can’t identify the yeasts at the species level. So, their performance is needed to be reassessed. The VITEK 2 system allows species identification and antifungal susceptibility testing in a full-automated manner by using ID-YST cards. This is achieved in 15 h due to a sensitive fluorescence-based technology. So, the chance of good management of critical infections is increased with improvement of their fatality. The current study aimed at identification of yeast infection in immunocompromised cancer patients to the species level by a rapid |