الفهرس 
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Abstract
The present study was carried out in plant tissue Culture Laboratory of Vegetable and Floriculture Department, Faculty of Agriculture, Mansoura University, Egypt throughout the period from 2016 to 2017. The aim of this study is to improve the propagation of Impatiens balsamina, L. by an efficient protocol for in vitro propagation was successfully established through tissue culture technique, and studies the effects of different types and concentration of auxins and cytokinins on different parts of explants that derived from 21 days in vitro germinating seeds after surface sterilization. The best method for sterilization was by soaking seeds in ethanol (70%) for (60 Sec) followed by Sodium Hypochlorite (30%) for (10 min) that gave the highest percentage of seeds viability (Germination %) and seedling height of Garden Balsam red and pink.Cotyledonary node with sections of cotyledon explants was the best explants than other types of explants.Increased multiplication was through using cotyledonary node with sections of cotyledon explants on medium supplemented with BAP at 4.5 mg/l alone for eight weeks then subculturing for six weeks on the same medium (4.5 mg/l BAP alone); also it gave good number of roots with NAA.The best shoot length ,leaves number, roots length and number was obtained by sub culturing the explants on MS medium received 1.0 mg/l BAP + 0.5 mg/l NAA to MS without growth regulators after six weeks and didn’t need to elongation or rooting stage. Best callus size, volume and fresh weight produced by sub culturing the shoots from TDZ 3.0 mg/l medium to medium without growth regulators.TDZ can use efficiently with low concentrations and short periods of exposure. The tallest and the healthy roots number were obtained from full strength medium fortified with NAA at 0.1 mg/L.This protocol had five stages consisting of seed sterilization by ethanol (70%) for (60 Sec) followed by Sodium Hypochlorite (30%) for (10 min), multiplication stage on medium containing BAP 4.5 mg/L, subculture stage on the same medium, an elongation stage in presence of GA3 and a final rooting stage on medium with NAA. It lasted about 5 months to get regenerated plant from seedling-derived cotyledonary node with section of the cotyledon.