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العنوان
Bacterial Synthesis and characterization of Metals Nanoparticles and their Applications against some Phytopathogenic Bacteria =
المؤلف
Moustafa, Ayman Kamal,
هيئة الاعداد
باحث / Ayman Kamal Moustafa
مشرف / Alia A. Shoeib
مشرف / Sahar Abd El-Fatah Zaki
مشرف / Nader Abd El-Wahab Ashmawy
الموضوع
Plant diseases.
تاريخ النشر
2018.
عدد الصفحات
108 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
العلوم الزراعية والبيولوجية
الناشر
تاريخ الإجازة
8/11/2018
مكان الإجازة
اتحاد مكتبات الجامعات المصرية - Plant Pathology
الفهرس
Only 14 pages are availabe for public view

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Abstract

Plants exposed for attacking of a large spectrum of pathogens, there are over 200 plant pathogenic bacterial species that cause diseases to plants making it easy for the death and damage. The using of nanomaterials in plant diseases management is a novel and fancy approach and alternative to traditional methods for diseases management that may prove very effective in future with the progress of application aspect of nanotechnology in agriculture.
The aim of present work was to study preparation and characterization of various types of nanomaterial using bacterial strains isolated from Egyptian sampling sites, and studying their effect on some species that cause bacterial plant diseases.
The most important results of the present study could be summarized in the following:
1. Many water samples from different locations in Egypt from the following places: -Industrial waste water in Damietta - Port of Ras Sedr (sea water) - Gulf of Suez (sea water) -Abu Qir bay (sea water) - Tlombat Al-Max (wastewater) - Eastern port Alexandria (sea water) - Mariout Lake (lake water) - Port of Mount of oil (sea water). Were used for isolation of bacterial isolates can reduce some metals to nanomaterials.
2. The chemical analysis of the collected samples showed a group of inorganic pollutants from heavy metals such as cadmium, lead, iron, copper and zinc.
3. Four bacterial isolates with dark colony or halo zone were isolated from the collected water samples and screened as metal reducer.The four selected isolates were identified by Biochemical and physiological tools, as well as 16S rRNA nucleotide sequence, the selected bacterial isolates were identified as Enterococcus thilandicus for Copper nanoparticles production, Pseudomonas putida for Iron nanoparticles production, Marinobacter hydrocarbonoclasticus for Cobalt nanoparticles production, Pseudomonas geniculate for Zinc nanoparticles production.
4. Nitrate reductase enzyme was assayed for the selected bacterial isolates which demonstrated that strains with maximum enzyme production was Pseudomonas putida (1400 U/ml), and Marinobacter hydrocarbonoclasticus 850 U/ml.
5. The produced nanoparticles were characterized by using UV-Vis spectroscopic, the absorbency peaks for iron, copper, cobalt, and zinc nanoparticles were observed at 233 nm, 569 nm, 530 nm and 230-250 respectively.
6. TEM analysis were elucidate that the synthesis of metals nanoparticles intercellular in cytoplasm and periplasmic space addition to metals nanoparticles recorded different size (iron nanoparticles: 1-4 nm, copper nanoparticles: 8-14 nm, cobalt nanoparticles 9-20 nm and zinc nanoparticles 4-13 nm).
7. The atomic percentage of the metals nanoparticles revealed strong signals in the iron region with 59 %, copper with 41 %, cobalt 47 and zinc with 46 %.