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العنوان
Antibiotic residues in raw milk sold at local markets=
المؤلف
Mahmoud ,Nesma Esmail Ahmed.
هيئة الاعداد
باحث / نسمة اسماعيل أحمد
مشرف / أحلام أمين اللبودى
مشرف / حسين صبحى أبو المكارم
مناقش / سلوى أحمد محمد
مناقش / عمرو عبد المؤمن عامر
الموضوع
Milk Hygiene. التفريع إن وجد
تاريخ النشر
2020.
عدد الصفحات
055 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البيطري
تاريخ الإجازة
23/1/2020
مكان الإجازة
جامعة الاسكندريه - كلية الطب البيطرى - الرقابة الصحية على الألبان
الفهرس
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Abstract

Present work studied the incidence of antibiotic residues including (Oxytetracyclin, Penicillin G and Cloxacillin) in raw milk sold at local markets and dairy shops in Alexandria governorate. In addition, comparing positive samples with Maximum residual limits of international standards and calculated acceptable daily intake for each antibiotic. Therefore, 80 samples of raw milk of different species including (cow, buffalo, camel and sheep) collected from different local markets in Alexandria city.
The obtained results summarized in the following items:
7.1. Oxytetracyclin residues in examined raw milk samples of different animals:
7.1.1. Detection rate of oxytetracycline residue in examined raw milk samples by screening FPT and HPLC were 35 and 30% in cow`s milk, 25 and 30% in buffalo, 20 and 15% in camel and 40 and 30% in sheep`s milk.
7.1.2. Mean values of oxytetracycline residue (ppb) detected by HPLC technique were 97.9± 28.1, 85.5± 25.9, 48.4 ± 28.0 and 122.9 ± 29.2 ppb in cow, buffalo, camel and sheep milk samples, respectively.
7.1.3. Positive samples above MRL were 15, 10, 5 and 25% in examined cow, buffalo, camel and sheep milk samples, respectively.
7.1.5. Mean values of calculated daily intake based on consumption of 250 ml raw milk/day lower than acceptable daily intake.
7.2. Penicillin G residues in examined raw milk samples of different animals:
7.2.1. Detection rate of Penicillin G residue in examined raw milk samples by screening FPT and HPLC were 30 and 25%, 15 and 20%, 10 and 10% and 35 and 25% in examined cow, buffalo, camel and sheep milk samples, respectively.
7.2.2. Mean penicillin residue G concentrations were 8.86± 3.60, 7.83± 4.73, 1.95 ±0.95 and 10.2±3.40 ppb in examined cow, buffalo, camel and sheep milk, respectively.
7.2.3. Positive samples above MRL were 15, 10, 0 and 20% in examined cow, buffalo, camel and sheep`s milk samples, respectively.
7.2.4. Calculated daily intake based on consumption of 250 ml raw milk contaminated with Penicillin G residues /day/ person constituted 0.53, 0.46, 0.11 and 0.61% of ADI and therefore safe for human consumption.

7.3 Cloxacillin residues in examined raw milk of different animals
7.3.1. Detection rate of cloxacillin residue in examined raw cow, buffalo, and sheep milk samples by screening FPT and HPLC were 15 and 10%, 10 and 10%, 20 and 15%, respectively and failed to be detected in camel milk.
7.3.2. Mean cloxacillin residue concentrations were 39.3± 7.40, 21.5± 9.05, 0 and 37.6 ± 9.91ppb in examined cow, buffalo, and camel and sheep milk samples, respectively with respective incidence of 10, 10, 0 and 15%.
7.3.3. Positive samples above MRL were 10, 5, 0 and 10% in examined cow, buffalo, camel and sheep milk samples, respectively.
7.3.4. Calculated daily intake based on consumption of 205 ml raw milk contaminated with Cloxacillin residues /day/ person constituted 1.01, 0.54, 0 and 0.96 % of ADI and therefore safe for human consumption.
7.4. Samples contaminated with only one antibiotic were 15,20,5 and 20%, samples; contaminated with two antibiotic residues were 20, 20,10and 20% respectively, and the percent of samples contaminated with three antibiotics residues were 5, 0, 0 and 5%.