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Abstract Gluconacetobacter diazotrophicus and Bacillus subtilis have been utilized mainly as plant-growth promoting bacterium to mitigate the dependence on application of chemical fertilizers in agriculture and introduce them into the approach of sustainable climate smart agriculture to meet our food security demands. Our present research project intended to study the impact of G. diazotrophicus (a strain proprietary to Azotic Technologies Ltd, BioCity, Nottingham, UK) on growth improvement and yield productivity of different crops including tomato and wheat. The colonization of plants with G. diazotrophicus was studied and generation of G. diazotrophicus-nifD- mutants was conducted to examine its role in nitrogen fixation. |