الفهرس 
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Abstract
This work was carried out to study the bacteriological importance of Cronobacter sakazakii as a potential foodborne emerging pathogen involved in severe illness and deaths in humans, especially neonates.
Isolation of Cronobacter spp. in different samples collected from Zagazig city, Sharkia province, Egypt:
• A total of 100 samples [PIF milk (n=55), PIF food (n=15), milk powder (n=15) and milk powder products (n=15) were collected and subjected to bacteriological examination for the presence of Cronobacter.
• Six samples out of 100 examined (6%) were found positive for Cronobacter spp.
• The isolation rates were 5% in PIF milk and 1% in PIF food
• The identity of the isolated organism was confirmed as Cronobacter spp. by subjecting the bacteriologically positive samples to PCR technique using 16S rRNA species specific primers.
• Cronobacter specific 16S rRNA was detected respectively in 3/5 and 1/5 of bacteriologically positive PIF milk and PIF food examined.
• All positive 16S rRNA (n=4) were examined for the presence of C. sakazakii.
• C. sakazakii were confirmed in an isolate from 3 isolates of PIF milk and one of PIF food.
Molecular characterization and detection of virulence genes (ompA and zpx) in C. sakazakii.
• The confirmed Cronobacter (n=4) were examined for harboring the virulent genes ompA and zpx.
• ompA gene was detected in 2 identified C. sakazakii isolates. While, 3 Cronobacter isolates were negative for ompA. However, the zpx virulence associated gene was identified in one C. sakazakii isolate.
Antibiotic susceptibility of Cronobacter isolates to different antibiotics:
• All Cronobacter isolates in the current study were subjected to antibiotic susceptibility test to 8 antibiotics of different groups.
• For C. sakazakii, all C. sakazakii isolates were sensitive to ciprocin, while 75% of the isolates were resistant to rifampicin and streptomycin.