الفهرس 
Introduction and review of literatureOnly 14 pages are availabe for public view
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Abstract
Oral squamous cell carcinoma is an aggressive epithelial malignancy with around 270,000 new cases occurring annually. OSCC has one of the lowest survival rates and remains unaffected despite recent therapeutic advances. Diagnostic delay has been shown to be a significant factor in disease progression. Screening for OSCC is easy with a simple visual examination of the oral cavity as compared to other malignancies (breast, prostate and colon) which require more elaborate screening tests.
Cancer chemoprevention is the use of natural, synthetic or biological chemical agents to reverse, suppress or prevent carcinogenic progression. Curcumin and its nanoparticles considered one of these natural drugs that can be used in management of different diseases.
Curcumin is a polyphenol extracted from the herb Curcuma longa. Curcumin is widely used in traditional Chinese medicine and in the food industry. It also has demonstrated several types of biological and pharmacological activities, including anticancer, anti-inflammatory and antioxidant properties.
To increase the retention time of Curcumin in the body, various formulation techniques have been applied. For example, PLGA is the material generally used for Nano-formulation since it is biodegradable and has been approved by the U.S. Food and Drug Administration for pharmaceutical application. Recent in vitro studies have revealed that Curcumin inhibition of cancer cell growth was effected by Curcumin-loaded PLGA nanoparticles due to enhanced uptake by cells.
In this study, we investigate the cytotoxic effect of Curcumin and Nanocurcumin on HEP-2 cell line using MTT assay and morphometric analysis. The change in cell cycle distribution of HEP-2 cell was detected using flow cytometry. By increasing Curcumin and Nanocurcumin concentrations, total cell death (apoptosis and necrosis) have been shown.
The results showed that Curcumin and Nanocurcumin have cytotoxic effect on viability of HEP-2 cells using MTT assay. The morphometric analysis confirmed the results of MTT assay, as there was a decrease in nuclear area factor in a dose dependent manner.
Curcumin and Nanocurcumin may be important economically and would allow a relatively inexpensive cancer treatment. Preclinical investigations are needed for further elucidate the benefits and antitumor properties.
The present outcomes from this study are going in same way with many documentations. These reports have indicated that Curcumin and Nanocurcumin can inhibit cell growth and reduce proliferation of tumor cells.