الفهرس 
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Abstract
Introduction :To evaluate the effect of ozonated water with and without nano-hydroxyapatite (NHA) paste on remineralizing artificially created initial enamel caries around orthodontic brackets using polarized light microscopy and Vickers microhardness tester. Material and Methods : A total of 120 extracted human premolar sound teeth were collected and coded from 1 to 120. Metallic brackets were bonded to the buccal surface of the teeth, then specimens were divided into 4 groups following different remineralization regimen: ozonated water, ozonated water + 10 % NHA paste, 10 % NHA paste alone, and control (saliva). The surface microhardness (SMH) was measured using HVS-50 Digital Display Vickers microhardness tester at baseline. The specimens were subjected to demineralizing solution to create initial enamel caries and enamel SMH was recorded. The specimens were subjected to pH cycling model then enamel SMH was recorded. The results were validated qualitatively using polarized light microscope (PLM).The results were analyzed using repeated measures, one-way ANOVA with post hoc multiple comparisons at α =0.05 level. Results : A statistically significant difference was found between all treatment groups and control one (P<0.001). The greatest depth of remineralization was found in ozone-treated group then ozone + NHA then NHA alone. Ozone-treated group exhibited maximum remineralization depth under the PLM. Conclusion : Ozonated water remineralization was the most effective of all. The remineralizing ability of ozone is seen with and without an adjunct remineralizing agent. Additionally, ozonated water enhanced the remineralizing potential of NHA.