الفهرس 
Introduction
Aim of the studyOnly 14 pages are availabe for public view
 |  | from | 97 |  |  |
| | | from | 97 | | |
Abstract
GCs play an important role in supporting the nuclear and cytoplasmic maturation of oocytes resulting in oocytes that are capable of undergoing normal fertilization and subsequent embryonic development. They also protect the oocyte against cell damage induced by oxidative stress by acting as a free radicals scavenger, and are involved as a key element through its collaborative relationship with theca cells in production of estradiol. Oxidative stress during in vitro culture due to exposure to hypoxic status leads to GCs apoptosis along with metabolic and steroidogenic insufficiency. Therefore, the trials to enhance the steroidogenic, metabolic and antioxidant efficacy of GCs are important in improving the developmental capacity of oocyte under the in vitro culture conditions.
KP acts mostly centrally through HPG axis via induction of GnRH secretion, so it plays an important role in reproduction. However, a lot of evidences points to presence of KP and its receptors in multiple ovarian structures giving a driving force for the idea that its potential peripheral effect is independent on the interplay ofneurohormonal circuits. With the exception of a study on chicken ovarian granulosa cells, up to the knowledge of the researcher, the majority of experiments on the ability of KP to induce E2 and P4 secretion were performed in vivo directing the attention towards the evaluation of its effects on BGCs especially in relation to variability in the size of follicles from which they are collected.
A wide array of evidences in the literature denote the antioxidant and cytoprotective properties of KP in the in vivo experimental models, however whether KP has the same properties in BGCs is the question that remain to be answered.
To shed the light on these unexplored areas of research, this study was designed to highlight the dose-dependent modulating effect of KP-10in the cultured BGCs of different sized follicles on steroid secretory potential and metabolic and antioxidant efficacy.
Following preparation of BGCs monolayer in this study, GCs were divided according to size of the follicles from which they were obtained into two main groups, cells from small follicles less than 8 mm in diameter and cells from large follicles more than 8 mm in diameter. BGCs collected from the small sized follicles were divided into four groups; control group (CS) received no treatment; KP(I)S group was exposed to KP at a dose of 10-8M; KP(II)S group was exposed to KP at a dose of 10-7 M; KP(III)S group was exposed to KP at a dose of 10-6M. BGCs collected from the large sized follicles were divided into four groups; control group (CL) received no treatment; KP(I)L group was exposed to KP at a dose of 10-8M, KP(II)L group was exposed to KP at a dose of 10-7 M; KP(III)L group was exposed to KP at a dose of 10-6M. After 24 hours from addition of KP, E2 , P4 , glucose, lactate and NO levels were measured in the spent media ,while LDH, TC, TAC and LPO were measured in GCs.
This study showed the following:
1. E2 level in the spent media of BGCs derived from small and large sized follicles incubated with KP-10 at all the examined doses was significantly higher than its level in the control groups with exception of KP(I)L group.
2. P4 level was significantly increased in the spent media from small sized follicles when incubated with KP-10 at the all examined doses compared to the control.
3. There was a reduction in E2 level of KP(III)S group in comparison with KP(I)S and KP(II)S groups and in P4 level of KP(III)S group in comparison with KP(I)S group and also that of KP(II)L and KP(III)L groups in comparison with KP(I)L group.
4. A significant reduction was observed in glucose level in the treated small sized follicle groups and KP(I)L group in comparison with the corresponding control groups. On the other hand, a significant elevation was noticed in the glucose level of KP(II)L and KP(III)L groups compared to CL group
5. There was a significant reduction in the lactate level of all treated groups except KP(I)S relative to the control groups.
6. KP(II)S and KP(III)L groups were characterized by a significant increase in LDH activity in comparison to control group.
7. A significant decrease was found in TC level of KP(I)S and KP(II)S groups compared to the control one. On the other hand, TC level of KP(I)L,KP(II)Land KP(III)L groups showed a significant increase compared to their control groups.
8. In comparison with the corresponding control groups, TAC level of KP(I)L and KP(III)L groups showed a significant elevation, while those of KP(I)S , KP(II)S and KP(II)L groups showed a significant reduction.
9. LPO level in KP(I)S, KP(I)L, KP(II)L and KP(III)L groups was significantly lower than that of the corresponding control groups