الفهرس 
Kidney structure and functionOnly 14 pages are availabe for public view
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Abstract
Recent clinical observational studies claimed that long term use of proton pump inhibitors (PPIs) might lead to kidney injury that might progress to end-stage renal disease.
The aim of the work was to verify these claims by an in vivo experimental study in the adult male albino rat by using omeprazole drug.
Forty two adult male albino rats were divided into four groups: Control group (I) in which rats were not administrated any treatment, in groups Ib,Ic and Id only distalled water administrated.
In groups IIa, IIb & IIc rats received daily oral omeprazole in dose of 0.75 mg per kg. for 2, 4, and 6 weeks successively. At the end of experiment, blood samples were collected for serum creatinine and blood urea nitrogen (BUN) measurement. Then animals were sacrificed and kidney specimens were processed into paraffin blocks, sectioned and stained with H&E, Masson’s trichrome & PAS.
Stained sections and image analysis were used to count vacuolated tubular cells, tubular cells with pyknotic nuclei, tubular casts and area precent of collagen fiber deposition then data was subjected to statistical analysis.
Histological examination of renal sections from omeprazole treated groups showed segmental kidney injury in renal corpuscle, renal tubules and sever vascular congestion with inflammatory cell infiltrate in renal interstitium.
Thickening of basement membrane was seen by PAS, while progressive increase in collagen fiber deposition was detected by Masson’s trichrome stain. Morphometry, image analysis statistical analysis confirmed histological findings.
Statistical significant increase in number of vacuolated cells, pyknotic nuclei, hyaline casts and area percent of collagen fibers deposition was found compared with control group.
Clear deterioration of renal function tests was observed across the 3 time points of the study compared with the control with highly significant rise of serum creatinine in 4 & 6 weeks group compared with the control. Results of the study were directly propionate with duration of usage of PPIs and so the worst damage was in the 6 week group (IIc).