الفهرس 
Effect of Sodium Azide on vegetative growth and glycoside contents of Stevia rebaudiana grown in the field and in its calli.Only 14 pages are availabe for public view
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Abstract
One field experiment and one in vitro experiment were carried out during the years of of 2013 and 2014 at Sabahia Agriculture Research Station, Alexandria. Egypt Agriculture Research Center (A.R.C). The objective of this research was to study the effect of different concentrations of the chemical mutagen Sodium Azide (SA) on the morphological characteristics and chlorophyll, stevia sweeteners contents of Stevia rebaudiana plant as well as the possibility of mutation induction.The characters studied during in vivo experiments were: Plant survival percentage, plant height, number of leaves, number of branches, leaf area, fresh and dry leaves weights, total leaf chlorophyll contents (a, b and a+b), leaf carotenoid content, induced variations, stevia sweeteners contents and Peroxidase isozyme.The characters studied during in vitro experiment were:Growth of callus was measured in terms of number of callus, percentage of callus induction (%), fresh and dry weight of callus (g), callus stevioside contents.5. I. Field (In vivo) experiment The M1 generation On April 21, 2013 and April 20, 2014 in the first and second seasons respectively, on divided plants (20 – 25 cm) long were sown in plastic pots (20 cm) filled with a soil mixture consisting of 1 : 1 part of sand and clay. A randomized complete block design with four replicates were used. Each replicate contained 8 treatments (0. 0.10, 0.15, 0.20, 0.25, 0.30, 0.35 and 0.40 %) sodium azide concentrations. Each treatment (experimental unit) represented by five pots. Sodium Azide concentrations were prepared and applied using application method (soil drench). A single application of every treatment was applied on June 2, 2013 and June 1, 2014 in the first and second seasons respectively. 50 ml. sodium azide solutions per pot was added, plants did not receive irrigation for two days before and after mutagen applications. Treated plants were irrigated 3 times a week and fertilized whenever needed.At the end of the season the plants had been cut and then data recorded.The M2 generation The same previous experiment was carried out on April 20, 2014 and April 19, 2015 for the first and second seasons, respectively. The plants from all treatments were taken from the previous generation M1 without any treated mutagens.