الفهرس 
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Abstract
Monosodium glutamate is one of the food additives that
are widely used in many foods as a flavor enhancer. It
generated much controversy about its safety usage.
The aim of this work was to study the effect of
monosodium glutamate on the testis structure of the prepubertal
and adult albino rats.
The rats were divided into three groups, six animals each:
group I (control group): The rats were divided into two
subgroups: group 1A (Prepubertal rats) and group 1B (Adult
rats). They were given the same amount of normal saline as the
treatment groups.
group II: Monosodium glutamate treated prepubertal
rats aged 4 weeks. The rats in this group were given 4mg\gm
body weight MSG dissolved in normal saline daily using rat
gavage needle for 14 days.
group III: Monosodium glutamate treated adult rats
aged 12 weeks. The rats received the same dose as group two.
At the end of the experiment the animals were sacrificed
and the testis was dissected. Paraffin blocks were prepared
stained and with Haematoxyline and Eosin. Semi-thin sections
Summary and Conclusion
70
were prepared and stained with Toluidine blue. Ultrathin
sections were prepared, cut and stained with uranyl acetate and
lead citrate
Results:
group IA: Microscopic examination of cross sections of
testis showed oval or rounded seminiferous tubules .The
different spermatogenic cells were arranged in (5 – 8 layers).
The spermatogonia were seen followed by primary
spermatocytes then the rounded Spermatids with their
acrosomal caps on one side of the nucleus. Examination of the
ultrathin sections showed that the primary spermatocytes
showed a large ovoid nucleus. The nucleus was surrounded by a
rim of cytoplasm. Variable sized mitochondria were observed
near the nucleus and few cisterns of rough endoplasmic
reticulum. The rounded spermatids showed a centrally located
nucleus. The acrosomal cap was noticed as a dark
hemispherical zone flattened over one third of the nuclear
surface. The cytoplasm of these cells revealed the presence of
peripherally situated mitochondria.
group IB: Microscopic examination of the sections of
the control adult rat testis showed multiple oval or rounded
seminiferous tubules. The sperms were seen within the lumen
of the tubules. There was increase in number of elongated
spermatids and sperms. The primary spermatocytes showed
large rounded nuclei. Mitochondria were observed near the
Summary and Conclusion
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nucleus and few cisterns of rough endoplasmic reticulum .The
spermatids possessed a spherical or oval nucleus, over some
nuclei the acrosomic cap occupied almost one third of the
nuclear diameter.
group II: Microscopic examination of stained cross
sections of testis showed disturbed architecture of some
seminiferous tubules. The tubules showed focal depletion of
spermatogenic cells and disorganisation of germinal epithelium
(G). Sloughed cells were noticed in the lumen. Examination of
ultrathin sections showed that spermatocytes and spermatids
showed interrupted or poorly defined nuclear membrane
disrupted mitochondrial cristae and dilated smooth endoplasmic
reticulum.
group III: Microscopic examination of the stained cross
sections of treated adult rat testis showed shrunken
seminiferous tubule with moderate depletion of the
seminiferous tubule from the spermatogenic cells in comparison
to the control. The lumen showed sloughed cells. Examination
of ultrathin sections showed spermatocytes with poorly defined
cytoplasmic organelles. Although a few mitochondria were
found scattered around the cytoplasm, they showed disrupted
cristae. Nuclear degeneration was evident in the spermatocytes
as some cells showed irregular nuclear membrane. The rounded
spermatids were affected some cells had small, dark nuclei,
cytoplasmic vacuolization was observed and the cytoplasmic
organelles couldn’t be identified.