الفهرس | Only 14 pages are availabe for public view |
Abstract This work was conducted to study the effect of Aeromonas hydrophila among catfish (Clarias gariepinus) and their associated hematological, biochemical, immunological, pathological and antioxidant changes with trial for control using natural immunostimulant (Biogen and Ropadiar). A total of 120 catfish (Clarias gareipinus) were randomly assigned to 6 treated groups each of 20 as follows: G1 as the control; G2 received 2 gm Biogen supplement; G3 received 3 ml Ropadiar; G4 infected by A. hydrophila only; G5 supplied by 2 gm Biogen and infected with A. hydrophila; G6 supplied by 3 ml Ropadiar and infected with A. hydrophila. Clinicopathological investigation showed significant decrease in RBCs, Hb, PCV% and a significant increase in WBCs and lymphocyte in infected non-treated and infected treated groups. A significant increase in erythrocytic parameters in Biogen treated group while non-significant change was observed in Ropadiar treated groups. Serum ALT, ALP, Urea and glucose showed a significant increase in infected group, while infected treated groups showed a significant decrease as compared with the infected group. Serum total protein and albumin showed a significant decrease with non-significant increase in globulin in infected group. While infected treated groups showed non-significant difference as compared with the infected group. Immunological investigation revealed a significant increase in IL1β and IL10 in infected group. While infected treated groups showed a significant decrease in IL1β and IL10 as compared with the infected group. Regarding antioxidant parameters, the serum MDA and NO level were significantly increased while the TAC levels was significantly decreased in infected group. Whereas, infected treated groups showed a significant decrease in MDA and NO with a significant increase TAC levels as compared with the infected group. The supplemented groups with Biogen.s and Ropadiar were insignificantly different in comparison with the control. The histopathology was fully reported in different groups. Conclusion: Dietary supplementation of Biogen and Ropadiar could improve the humoral defence mechanism and anti-oxidant response in fish, which in turn can aid in the control and prevention of A. hydrophila infection. |