الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Determination of time of injury and time of death are the most vital topics in forensic medicine.
Wound age determination is frequently requested in medicolegal problems to reconstruct certain events e.g. accidents, quarrels, and to decide whether the wounds are a result of single or split occasions. Also an accurate estimation of time of death is a vital for criminal law and civil problems.
The present work aimed at evaluating the efficacy of Cluster of Differentiation 14 (CD14) as reliable marker for estimating the wound age. Also to evaluate the efficacy of P53 and Bcl2 as reliable apoptotic markers for estimating the time passed since death.
The study was conducted on 80 autopsy cases referred to the Medicolegal Department of Ministry of Justice, at Alexandria Governorate, Egypt. Forty cases were victims exhibiting different types of wounds with a known post infliction time. Another forty dead bodies with known time of death were included to study the time of death.
Skin biopsies were obtained during autopsy from the center of each wound for determination of its age. Skin biopsies of the normal skin were taken from the autopsy incision from each cadaver for estimation of time of death.
Sections from biopsies were histologically examined by H&E. Immunohistochemical staining was done using CD14, P53 and Bcl2 antibodies and the staining density was evaluated semi-quantitatively.