الفهرس 
Klebsiella PneumoniaeOnly 14 pages are availabe for public view
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Abstract
Summary
As Gram-negative pathogens for example Klebsiella pneumoniae
are becoming increasingly resistant to the most commonly used
antibiotics and the extensive and irrationable use of broad-spectrum
antibiotics results in multidrug resistant strains, this study has revealed
the occurrence of plasmid mediated Amp-C β-lactamase producing
Klebsiella pneumoniae in many samples as (sputum, endotracheal
aspirate, pus from wounds, urine, and blood sample, bronchoalveolar
lavage).
Plasmid-mediated Amp-C genes are able to spread widely between
different members of Enterobacteriaceae within hospital settings,
leading to spread of resistance against a wide range of antibiotics and
failure of nosocomial infections treatment.
The objective of present study is to detect the prevalence of Amp-
C enzyme in several samples and to make a comparison between the
phenotypic tests with gold standard multiplex PCR in clinical isolates of
Klebsiella pneumoniae.
There were (840) isolates showed growth of gram-negative
organisms out of them (40%) (336) isolates were Klebsilla pneumoniae
proved by culture, biochemical reaction, then by application of disc
diffusion method there were (30%) (100) isolates multi-drug resistance
(MDR), A total of 100 Klebsiella pneumoniae isolates are collected
from different sites from different patients at Tanta University Hospital.