الفهرس 
Medicinal plants in traditional medicine:
Collection of plant seeds and preparation their extracts:Only 14 pages are availabe for public view
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Abstract
Plants are promising natural sources for biological active compounds, in addition, some of them have been used in treatment of some tumors. In re-cent decade’s number of natural chemicals extracted from medicinal plants as anti-cancer compounds such as taxol and vincristine, are used medically around the world (Wang et al., 2008). In traditional and alternative therapy, millions of people are using the medicinal plants for treatment of various diseases including cancer (Cragg and Newman, 2005). Medicinal plants are considered as potential sources of several chemical ingredients that used in drug discovery (Balunas and Kinghorn, 2005). Plant-derived products lead to reduce the chemical remedies in treatment and may reduce the ad-verse side effects of chemotherapy during the treatment (Iqbal et al., 2017). This study was carried out to evaluate the antioxidants and anticancer ac-tivities of some fruits seeds extract such as P. americana, P. armeniaca, P. domestica, O. europaea, H. thebaica and P. dactylifera. Human breast (MCF-7) and hepatic (HepG-2) cancer cell lines were used for in vitro anti-cancer assessments; in addition, ehrlich ascetic carcinoma (EAC) mouse model was used for evaluating the antitumor efficacy in vivo. Fruits were purchased from a local market in Tanta city, Egypt. Different seeds were ex-tracted, dried, crushed and then kept for further studies. 50 g of each seed powders were vigorously mixed with 500 ml 70% ethanol. The hydro-alcoholic extracts were dried and suspended in 0.9 % sterile saline for fur-ther processing. Qualitative analysis, total phenolic, flavonoids, saponin and anthocyanin were determined in the previously mentioned six extracts. Quantitative analysis, total antioxidants capacity (TAC) of extracts, DPPH scavenging activity of extracts, saponin content were also determined. Gas chromato-graph–mass spectrometry (GC-MS) analysis was carried out. Evaluation in vitro cytotoxic effects by human cell lines (MCF-7 and HepG-2) and cyto-toxicity assessment by MTT assay, ehrlich ascites carcinoma (EAC) cells were used. The study was further extended to address the efficacy of seed extracts as antitumor agents in vivo. To test the efficacy of these extracts on tumor model, EAC-cells were inoculated (2 × 106/mouse). After EAC-cells inoculation, mice were divided into different groups as following: group 1: control mice, group 2: tumor bearing mice (EAC) alone, group 3: EAC and injected with cisplatin (2 mg/kg/6 days), group 4: EAC and injected with P. americana, group 5: EAC and injected with P. armeniaca, group 6: EAC and injected with P. domestica, Group7: EAC and injected with O. euro-paea, group 8: EAC and injected with H. thebaica, group 9: EAC injected with P. dactylifera seeds extract. The extracts were injected as 100 mg/kg from day 1 to day 6 after 24 hours of tumor inoculation. On the day 14, mice were sacrificed.