الفهرس 
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Abstract
This work was performed to quantitatively determine minocycline residues in different tissues of chickens (kidneys, liver, muscles and fat) and sera following multiple oral doses of the drug using High Performance Liquid chromatography (HPLC). Moreover, the study aimed to estimate the withdrawal time of this drug in chicken tissues. The experiment was conducted on 25 chicks, Five samples (liver, kidney, breast muscle, fat and serum) were collected from each slaughtered bird (n=3) at six withdrawal periods (at 1st, 3rd, 7th, 14th, 21st and 28th day) after 24 h following the last oral medication dose for HPLC and biochemical investigation (liver and kidney function tests).
The Results:-1- Linearity existed within range of 0.025 and 5μg/gm with a correlation coefficient (r2=0.99982). The percentage recovery of spiked samples ranged from 95-98%. The limit of detection for minocycline was 0.0025μg/ml, while, LOQ (limit of quantification) was 0.01μg/ml.
2-Widespread distribution of minocycline in the tissue samples, which remained within the detectable limit till the 3rd day in all tested tissues. While in kidneys, minocycline remained till the 7th day following the last oral administration of the drug.
3-The highest minocycline residual level was detected in kidney followed by liver while the lowest level was recorded in muscle and fat samples.
4-Our detectable levels of minocycline residues in kidney, liver and muscle samples were lower than the established MRL of WHO/FAO.
5-Minocycline administration at recommended therapeutic dose (7 mg/kg BW) caused a transient significant influence the dynamics of tested biochemical indices (liver and kidney function tests) of broiler chickens then reached to safe levels during the withdrawal period at other time points. The results confirmed the presence of minocycline residues in chicken samples. Higher level of minocycline residues usually observed in kidney and liver rather than in muscle due to their role in metabolism and excretion of antibiotics. This may pose potential hazard to public health. Adhering to these precautions can prevents any adverse drug reactions and untoward residual effects. This may pose potential hazard to public health. Thus, it is recommended that rules should be taken to ensure observing proper withdrawal periods before marketing and drug control in veterinary use. In addition, a monitoring policy should be implemented to ensure the conformity of poultry meat sold in Egypt with international standards.