الفهرس 
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Abstract
Enterococcus is a commensal organism found in poultry and the human gastrointestinal tract. In recent years, Enterococcus has emerged as a major zoonotic and nosocomial pathogen.A total of 480 samples (70 from chickens, 30 from pigeon and 20 from ducks) were collected from (liver, heart, intestine and cloacal swabs) and also a total number of 120 samples (urine, pleural fluid, stool, hand wound and pus )from human were collected. The prevalence of Enterococcus isolation from poultry flocks was (53.3%) from poultry and from human (61.7%). The most predominant isolated Enterococcus species were E.faecium and E.faecalis.The antibiogram showed susctiblity of Enterococcus spp. to vancomycin. .The PCR identification of Enterococcus species was higher than results by conventional methods. Partial sequences of 16rRNA gene was applied to isolates from human and poultry.