الفهرس 
Effect of different bioactive material formulations on Streptococcus mutans activity testOnly 14 pages are availabe for public view
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Abstract
This study was conducted to evaluate between the effect of Biodentine and Glass ionomer restorative materials; on Streptococcus mutans activity and their effect on remineralization of the infrastructure of dentin.
Two hundred and twenty seven specimens were prepared throughout the whole study. For the streptococcus mutans activity test; a total of 32 specimens were prepared and equally divided into four main groups (8 specimens each) representing the four tested restorative materials (KetacᵀᴹFil Plus, ChemFilᵀᴹ Rock, PhotacᵀᴹFil Quickᵀᴹ and Biodentine®).
For the dentin remineralization test; a total of 195 specimens were prepared and then divided into two main groups, the control group (15 specimens) and the experimental group (180 specimens).
The experimental group was further divided into four subgroups (45 specimens) representing the four tested restorative materials applied to the dentin surface. Each restorative materials was prepared according to manufacturer instructions.
Streptococcus mutans activity testing was carried out by the Kirby-Bauer disc diffusion method according to CLSI recommendations 2014, while Dentin remineralization assessment (chemical analysis) was carried out using EDAX attached to SEM to identify the calcium and phosphorus levels in dentin before and after application of the tested materials.
Results of the study showed that; the restorative material type, antibiotic type and their interaction had a significant effect on streptococcus mutans activity.
Erythromycin with ketac fil showed the highest mean value (25.62 ±7.28) for the effect of different tested materials and antibiotics on streptococcus mutans activity, while the clindamycin with biodetine showed; the lowest mean value.
For dentin remineralization chemical assessment (chemical analysis); material type, time intervals ( 24 hours, one month, three months) and their interaction had a significant effect on both calcium and phosphorus weight percents (wt%).
Highest mean calcium levels (wt%) was recorded for the biodentine when stored for three months (74.35 ± 3.47), while the lowest mean value was recorded for photac fil quick when stored for 24 hours.
Highest mean phosphorus levels was recorded for photac fil quick when stored for 24 hours (34.67 ± 9.17), while the lowest mean value was recorded for biodentine when stored for three months (25.64 ± 3.47).
Thus under the limitation of the current study, the following conclusions could be derived:
1) Restorative material type and antibiotic type had a significant effect on streptococcus mutans activity as well as on dentin remineralization.
2) Erythromycin showed the highest positive effect on the streptococcus mutans activity with the different tested restorative materials.
3) Incorporation of zinc in glass ionomer composition had the potential to increase its antibacterial activity. However; it did not significantly affect the calcium and phosphorus ions levels in dentin even with storage in distilled water upto three month.
4) Calcium silicate based materials have the ability to enhance dentin remineralization through increased calcium ion levels in dentin when stored up to three months in distilled water. On the other hand; it showed a lower ability with phosphorus.
5) Resin modified glass ionomer has the potential to increase phosphorus ion levels in dentin when stored up to three months in distilled water. However; it showed a lower ability with calcium.